Sequence of the preprotoxin dsRNA gene of type I killer yeast: multiple processing events produce a two-component toxin
UMass Chan AffiliationsDepartment of Microbiology and Physiological Systems
Department of Molecular Genetics and Microbiology
MetadataShow full item record
AbstractThe preprotoxin gene of the 1.9 kb M1 dsRNA genome from type I killer yeast has been sequenced employing a partial-length cDNA derived from an in vivo transcript. A single open reading frame, commencing with AUG at M1 dsRNA bases 14-16, terminates with UAG at 963-965 and codes for a 316 amino acid protein, believed to be identical to the 34 kd preprotoxin species, M1-P1, synthesized by in vitro translation of denatured M1 dsRNA. N-terminal sequencing of M1-P1 confirms this prediction. Secreted toxin is shown to consist of two dissimilar, disulfide-bonded subunits, alpha and beta, of apparent size 9.5 and 9.0 kd, respectively, whose N-terminal sequences are also found in the predicted preprotoxin sequence. Its proposed domains consist of delta, a 44 amino acid N-terminal segment, followed by alpha and beta, which are separated by gamma, a large central glycosylated segment. Processing sites, domain functions, and the potential role of gamma in immunity are discussed.
Cell. 1984 Mar;36(3):741-51.
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/36466