Virus-like particle capsid proteins encoded by different L double-stranded RNAs of Saccharomyces cerevisiae: their roles in maintenance of M double-stranded killer plasmids
UMass Chan Affiliations
Department of Microbiology and Physiological SystemsDepartment of Molecular Genetics and Microbiology
Document Type
Journal ArticlePublication Date
1984-12-01
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Show full item recordAbstract
The plasmid determinants of killer phenotypes in type K1 and K2 killer yeast cells are the 1.9-kilobase (kb) M1 and 1.7-kb M2 double-stranded RNAs (dsRNAs), respectively. These are dependent for their maintenance and encapsidation, in Saccharomyces cerevisiae virus ScV-M1 or ScV-M2 virus-like particles, on the capsid provided by one of a group of moderately related 4.7-kb dsRNAs called LA. The L1A and L2A dsRNAs found in naturally isolated K1 and K2 killers encode 88-kilodalton VL1A-P1 and 86-kilodalton VL2A-P1 capsids, respectively. These are competent for encapsidating homologous LA dsRNAs as well as M dsRNAs. Most strains of S. cerevisiae, including killers, contain one of a second group of closely related 4.7-kb dsRNAs called LBC. These encode their own 82-kilodalton capsid protein, VLBC-P1, which, at least in strains containing only LBC, encapsidates homologous dsRNA in ScV-LBC virus-like particles. In a K1 killer strain containing both L1A and LBC, ScV-M1 particles contain only VL1A-P1. In such strains it is probable that each virus-like particle contains a single capsid type and that each L dsRNA is encapsidated by a homologous capsid.Source
Mol Cell Biol. 1984 Dec;4(12):2818-27. doi: 10.1128/mcb.4.12.2818. Link to article on publisher's site
DOI
10.1128/mcb.4.12.2818Permanent Link to this Item
http://hdl.handle.net/20.500.14038/36469PubMed ID
6396508Related Resources
ae974a485f413a2113503eed53cd6c53
10.1128/mcb.4.12.2818