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dc.contributor.authorBlom, Hans
dc.contributor.authorMorse, Robert
dc.contributor.authorMandelkorn, J.
dc.contributor.authorArnaud, M.
dc.contributor.authorWarburg, R.
dc.contributor.authorTipper, Donald J
dc.date2022-08-11T08:09:18.000
dc.date.accessioned2022-08-23T16:26:01Z
dc.date.available2022-08-23T16:26:01Z
dc.date.issued1987-08-01
dc.date.submitted2019-05-17
dc.identifier.citation<p>J Gen Microbiol. 1987 Aug;133(8):2237-46. doi: 10.1099/00221287-133-8-2237. <a href="https://doi.org/10.1099/00221287-133-8-2237">Link to article on publisher's site</a></p>
dc.identifier.issn0022-1287 (Linking)
dc.identifier.doi10.1099/00221287-133-8-2237
dc.identifier.pmid2450963
dc.identifier.urihttp://hdl.handle.net/20.500.14038/36473
dc.description.abstractIn Bacillus subtilis the alpha, beta, gamma and delta components comprise 80-90% of the total acid-soluble spore proteins (ASSPs). Sequence analysis demonstrates that alpha and beta share 32 of their first 36 amino acids and are closely related to the A and C ASSPs of Bacillus megaterium spores, confirming the results of analysis of their cloned genes. Despite the difference in apparent size of gamma and delta, they have identical N-terminal sequences (37 residues). Unless gamma and delta derive from very recently duplicated genes, it appears that gamma is derived from delta, either in vivo or during isolation. Although the sequenced regions of gamma and delta have no homology to alpha and beta, outside of the previously recognized pentapeptide recognition sequence for the spore endopeptidase, they share 10 and 15 residue peptides flanking this sequence with ASSP B of B. megaterium, but in reverse order. At least two groups of ASSPs have, therefore, been conserved between B. subtilis and B. megaterium: the multigene AC alpha beta family and the B gamma (delta) group. Sequence conservation in each group implies selection for functions in addition to storage. Both the alpha and beta components of B. subtilis ASSPs and their mRNAs are located in the forespore compartment of cells at t5.5 of sporulation, the time of most rapid ASSP synthesis. The sizes of these transcripts (250-350 bp) and their ability to direct the in vitro synthesis of ASSPs of mature size, indicate that genes for these ASSPs are monocistronic, consistent with dispersed map location. Synthesis of ASSPs is, therefore, coordinately controlled by selective transcription in the forespore.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=2450963&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1099/00221287-133-8-2237
dc.subjectMicrobiology
dc.subjectPhysiology
dc.titleThe major acid-soluble proteins of Bacillus subtilis spores: partial amino acid sequence and forespore location of their mRNAs
dc.typeJournal Article
dc.source.journaltitleJournal of general microbiology
dc.source.volume133
dc.source.issue8
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/maps_pubs/37
dc.identifier.contextkey14524120
html.description.abstract<p>In Bacillus subtilis the alpha, beta, gamma and delta components comprise 80-90% of the total acid-soluble spore proteins (ASSPs). Sequence analysis demonstrates that alpha and beta share 32 of their first 36 amino acids and are closely related to the A and C ASSPs of Bacillus megaterium spores, confirming the results of analysis of their cloned genes. Despite the difference in apparent size of gamma and delta, they have identical N-terminal sequences (37 residues). Unless gamma and delta derive from very recently duplicated genes, it appears that gamma is derived from delta, either in vivo or during isolation. Although the sequenced regions of gamma and delta have no homology to alpha and beta, outside of the previously recognized pentapeptide recognition sequence for the spore endopeptidase, they share 10 and 15 residue peptides flanking this sequence with ASSP B of B. megaterium, but in reverse order. At least two groups of ASSPs have, therefore, been conserved between B. subtilis and B. megaterium: the multigene AC alpha beta family and the B gamma (delta) group. Sequence conservation in each group implies selection for functions in addition to storage. Both the alpha and beta components of B. subtilis ASSPs and their mRNAs are located in the forespore compartment of cells at t5.5 of sporulation, the time of most rapid ASSP synthesis. The sizes of these transcripts (250-350 bp) and their ability to direct the in vitro synthesis of ASSPs of mature size, indicate that genes for these ASSPs are monocistronic, consistent with dispersed map location. Synthesis of ASSPs is, therefore, coordinately controlled by selective transcription in the forespore.</p>
dc.identifier.submissionpathmaps_pubs/37
dc.contributor.departmentDepartment of Microbiology and Physiological Systems
dc.contributor.departmentDepartment of Molecular Genetics and Microbiology
dc.source.pages2237-46


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