In vivo effect of thiolutin on cell growth and macromolecular synthesis in Escherichia coli
UMass Chan Affiliations
Department of Microbiology and Physiological SystemsDepartment of Microbiology
Document Type
Journal ArticlePublication Date
1974-09-01
Metadata
Show full item recordAbstract
Thiolutin reversibly inhibits growth and ribonucleic acid synthesis in Saccharomyces cerevisiae. It is now demonstrated that, at 5 mug/ml, thiolutin rapidly inhibits all incorporation of radioactive precursors into ribonucleic acid and protein in Escherichia coli, although the incorporation of deoxythymidine into deoxyribonucleic acid continues for some time. Concentrations of thiolutin of 5 mug/ml and above are bacteriostatic and do not lead to unbalanced growth, so that cell size remains constant. The antibiotic and its inhibitory effects are easily removed by washing, whereupon macromolecular synthesis and cell division resume unimpeded. These data are consistent with reversible inhibition of ribonucleic acid synthesis being the primary mode of action of thiolutin in E. coli, and suggest that thiolutin may be a useful tool for studies where such reversible inhibition is required.Source
Antimicrob Agents Chemother. 1974 Sep;6(3):304-10. doi: 10.1128/aac.6.3.304. Link to article on publisher's site
DOI
10.1128/aac.6.3.304Permanent Link to this Item
http://hdl.handle.net/20.500.14038/36494PubMed ID
15830477Related Resources
Rights
Copyright © 1974, American Society for Microbiology. Publisher PDF posted as allowed by the publisher's copyright policy at https://journals.asm.org/content/copyright-transfer-and-supplemental-material-license-agreement-2017.ae974a485f413a2113503eed53cd6c53
10.1128/aac.6.3.304