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    Inhibition of messenger ribonucleic acid synthesis in Escherichia coli by thiolutin

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    Authors
    Khachatourians, G. G.
    Tipper, Donald J.
    UMass Chan Affiliations
    Department of Microbiology and Physiological Systems
    Department of Microbiology
    Document Type
    Journal Article
    Publication Date
    1974-09-01
    Keywords
    Bacteriology
    Microbiology
    Physiology
    
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    Abstract
    Thiolutin, at concentrations of 5 to 40 mug/ml, inhibited the induced synthesis of beta-galactosidase in Escherichia coli CA8000. Thiolutin had no effect on the rate of in vitro hydrolysis of o-nitrophenyl-beta-d-galactoside by purified beta-galactosidase. Examination of the effects of thiolutin on the kinetics of appearance of beta-galactosidase in the presence and absence of rifampin in induced E. coli cells indicated that thiolutin interferes with the transcription process at the level of elongation of messenger ribonucleic acid (mRNA) chains. The data indicated that, in the presence of thiolutin, beta-galactosidase mRNA has a half-life of 1.6 min and that the first completed beta-galactosidase mRNA is produced about 1.5 min after induction. These data are consistent with estimates of transcription time and messenger half-life obtained by conventional means, and suggest that thiolutin does not affect translation of mRNA or its breakdown in vivo. After removal of thiolutin, cells fully regained the ability to be induced for synthesis of beta-galactosidase within 10 min, but mRNA which was incomplete at the time of thiolutin addition did not subsequently become functional.
    Source

    J Bacteriol. 1974 Sep;119(3):795-804. Link to article on publisher's site

    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/36495
    PubMed ID
    4604615
    Related Resources

    Link to Article in PubMed

    Rights
    Copyright © 1974, American Society for Microbiology. Publisher PDF posted as allowed by the publisher's copyright policy at https://journals.asm.org/content/copyright-transfer-and-supplemental-material-license-agreement-2017.
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