Cloning the Bacillus subtilis 168 aroC gene encoding dehydroquinase
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UMass Chan Affiliations
Department of Microbiology and Physiological SystemsDepartment of Microbiology
Document Type
Journal ArticlePublication Date
1984-12-01
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An approx. 14-kb Sau3A fragment of Bacillus subtilis DNA containing the aroC and ser-22 genes has been isolated. Gene aroC is expressed in both B. subtilis and Escherichia coli and appears to contain its own promoter, allowing complementation in B. subtilis. However, expression in E. coli is dependent on insert orientation, so the direction of transcription can be deduced. The level of dehydroquinase-specific activity, encoded by the cloned aroC gene, is raised 30- to 40-fold in both E. coli and B. subtilis. The clones are stable in both E. coli and B. subtilis but appear to have undergone several large deletions during their construction.Source
Gene. 1984 Dec;32(1-2):57-66. doi: 10.1016/0378-1119(84)90032-5. Link to article on publisher's site
DOI
10.1016/0378-1119(84)90032-5Permanent Link to this Item
http://hdl.handle.net/20.500.14038/36504PubMed ID
6442253Related Resources
ae974a485f413a2113503eed53cd6c53
10.1016/0378-1119(84)90032-5