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    Cloning the Bacillus subtilis 168 aroC gene encoding dehydroquinase

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    Authors
    Warburg, R. J.
    Mahler, Inga
    Tipper, Donald J.
    Halvorson, H. O.
    UMass Chan Affiliations
    Department of Microbiology and Physiological Systems
    Department of Microbiology
    Document Type
    Journal Article
    Publication Date
    1984-12-01
    Keywords
    Genetics and Genomics
    Microbiology
    Physiology
    
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    Link to Full Text
    https://doi.org/10.1016/0378-1119(84)90032-5
    Abstract
    An approx. 14-kb Sau3A fragment of Bacillus subtilis DNA containing the aroC and ser-22 genes has been isolated. Gene aroC is expressed in both B. subtilis and Escherichia coli and appears to contain its own promoter, allowing complementation in B. subtilis. However, expression in E. coli is dependent on insert orientation, so the direction of transcription can be deduced. The level of dehydroquinase-specific activity, encoded by the cloned aroC gene, is raised 30- to 40-fold in both E. coli and B. subtilis. The clones are stable in both E. coli and B. subtilis but appear to have undergone several large deletions during their construction.
    Source

    Gene. 1984 Dec;32(1-2):57-66. doi: 10.1016/0378-1119(84)90032-5. Link to article on publisher's site

    DOI
    10.1016/0378-1119(84)90032-5
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/36504
    PubMed ID
    6442253
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    Link to Article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1016/0378-1119(84)90032-5
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