Cloning the Bacillus subtilis 168 aroC gene encoding dehydroquinase
UMass Chan AffiliationsDepartment of Microbiology and Physiological Systems
Department of Microbiology
Document TypeJournal Article
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AbstractAn approx. 14-kb Sau3A fragment of Bacillus subtilis DNA containing the aroC and ser-22 genes has been isolated. Gene aroC is expressed in both B. subtilis and Escherichia coli and appears to contain its own promoter, allowing complementation in B. subtilis. However, expression in E. coli is dependent on insert orientation, so the direction of transcription can be deduced. The level of dehydroquinase-specific activity, encoded by the cloned aroC gene, is raised 30- to 40-fold in both E. coli and B. subtilis. The clones are stable in both E. coli and B. subtilis but appear to have undergone several large deletions during their construction.
Gene. 1984 Dec;32(1-2):57-66. doi: 10.1016/0378-1119(84)90032-5. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/36504