Show simple item record

dc.contributor.authorWarburg, R. J.
dc.contributor.authorMahler, Inga
dc.contributor.authorTipper, Donald J.
dc.contributor.authorHalvorson, H. O.
dc.date2022-08-11T08:09:18.000
dc.date.accessioned2022-08-23T16:26:09Z
dc.date.available2022-08-23T16:26:09Z
dc.date.issued1984-12-01
dc.date.submitted2019-07-15
dc.identifier.citation<p>Gene. 1984 Dec;32(1-2):57-66. doi: 10.1016/0378-1119(84)90032-5. <a href="https://doi.org/10.1016/0378-1119(84)90032-5">Link to article on publisher's site</a></p>
dc.identifier.issn0378-1119 (Linking)
dc.identifier.doi10.1016/0378-1119(84)90032-5
dc.identifier.pmid6442253
dc.identifier.urihttp://hdl.handle.net/20.500.14038/36504
dc.description.abstractAn approx. 14-kb Sau3A fragment of Bacillus subtilis DNA containing the aroC and ser-22 genes has been isolated. Gene aroC is expressed in both B. subtilis and Escherichia coli and appears to contain its own promoter, allowing complementation in B. subtilis. However, expression in E. coli is dependent on insert orientation, so the direction of transcription can be deduced. The level of dehydroquinase-specific activity, encoded by the cloned aroC gene, is raised 30- to 40-fold in both E. coli and B. subtilis. The clones are stable in both E. coli and B. subtilis but appear to have undergone several large deletions during their construction.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=6442253&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1016/0378-1119(84)90032-5
dc.subjectGenetics and Genomics
dc.subjectMicrobiology
dc.subjectPhysiology
dc.titleCloning the Bacillus subtilis 168 aroC gene encoding dehydroquinase
dc.typeArticle
dc.source.journaltitleGene
dc.source.volume32
dc.source.issue1-2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/maps_pubs/65
dc.identifier.contextkey14924930
html.description.abstract<p>An approx. 14-kb Sau3A fragment of Bacillus subtilis DNA containing the aroC and ser-22 genes has been isolated. Gene aroC is expressed in both B. subtilis and Escherichia coli and appears to contain its own promoter, allowing complementation in B. subtilis. However, expression in E. coli is dependent on insert orientation, so the direction of transcription can be deduced. The level of dehydroquinase-specific activity, encoded by the cloned aroC gene, is raised 30- to 40-fold in both E. coli and B. subtilis. The clones are stable in both E. coli and B. subtilis but appear to have undergone several large deletions during their construction.</p>
dc.identifier.submissionpathmaps_pubs/65
dc.contributor.departmentDepartment of Microbiology and Physiological Systems
dc.contributor.departmentDepartment of Microbiology
dc.source.pages57-66


Files in this item

Thumbnail
Name:
Publisher version

This item appears in the following Collection(s)

Show simple item record