Casein kinase 2-mediated phosphorylation of Brahma-related gene 1 controls myoblast proliferation and contributes to SWI/SNF complex composition
Authors
Padilla-Benavides, TeresitaNasipak, Brian T.
Paskavitz, Amanda L.
Haokip, Dominic T.
Schnabl, Jake M.
Nickerson, Jeffrey A.
Imbalzano, Anthony N.
UMass Chan Affiliations
Imbalzano LabNickerson Lab
UMass Metabolic Network
Department of Biochemistry and Molecular Pharmacology
Department of Pediatrics
Department of Biochemistry and Molecular Pharmacology
Document Type
Journal ArticlePublication Date
2017-11-10Keywords
Brg1SWI/SNF subunits
casein kinase 2
cell proliferation
chromatin regulation
chromatin remodeling
myoblast
phosphorylation
transcription
Biochemistry
Cell Biology
Cellular and Molecular Physiology
Molecular Biology
Metadata
Show full item recordAbstract
Transcriptional regulation is modulated in part by chromatin-remodeling enzymes that control gene accessibility by altering chromatin compaction or nucleosome positioning. Brahma-related gene 1 (Brg1), a catalytic subunit of the mammalian SWI/SNF chromatin-remodeling enzymes, is required for both myoblast proliferation and differentiation, and the control of Brg1 phosphorylation by calcineurin, PKCbeta1, and p38 regulates the transition to differentiation. However, we hypothesized that Brg1 activity might be regulated by additional kinases. Here, we report that Brg1 is also a target of casein kinase 2 (CK2), a serine/threonine kinase, in proliferating myoblasts. We found that CK2 interacts with Brg1, and mutation of putative phosphorylation sites to non-phosphorylatable (Ser to Ala, SA) or phosphomimetic residues (Ser to Glu, SE) reduced Brg1 phosphorylation by CK2. Although BRG1-deleted myoblasts that ectopically express the SA-Brg1 mutant proliferated similarly to the parental cells or cells ectopically expressing wild-type (WT) Brg1, ectopic expression of the SE-Brg1 mutant reduced proliferation and increased cell death, similar to observations from cells lacking Brg1. Moreover, pharmacological inhibition of CK2 increased myoblast proliferation. Furthermore, the Pax7 promoter, which controls expression of a key transcription factor required for myoblast proliferation, was in an inaccessible chromatin state in the SE-Brg1 mutant, suggesting that hyperphosphorylated Brg1 cannot remodel chromatin. WT-, SA-, and SE-Brg1 exhibited distinct differences in interacting with and affecting expression of the SWI/SNF subunits Baf155 and Baf170 and displayed differential sub-nuclear localization. Our results indicate that CK2-mediated phosphorylation of Brg1 regulates myoblast proliferation and provides insight into one mechanism by which composition of the mammalian SWI/SNF enzyme complex is regulated.Source
J Biol Chem. 2017 Nov 10;292(45):18592-18607. doi: 10.1074/jbc.M117.799676. Epub 2017 Sep 22. Link to article on publisher's siteDOI
10.1074/jbc.M117.799676Permanent Link to this Item
http://hdl.handle.net/20.500.14038/36626PubMed ID
28939766Related Resources
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Author’s Choice—Final version free via Creative Commons CC-BY license.Distribution License
http://creativecommons.org/licenses/by/4.0/ae974a485f413a2113503eed53cd6c53
10.1074/jbc.M117.799676
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Except where otherwise noted, this item's license is described as Author’s Choice—Final version free via Creative Commons CC-BY license.