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High-Throughput Screening of Tyrosine Kinase Inhibitor Resistant Genes in CML

dc.contributor.authorMa, Leyuan
dc.contributor.authorRoderick, Justine E.
dc.contributor.authorKelliher, Michelle A.
dc.contributor.authorGreen, Michael R.
dc.date2022-08-11T08:09:20.000
dc.date.accessioned2022-08-23T16:27:03Z
dc.date.available2022-08-23T16:27:03Z
dc.date.issued2016-09-01
dc.date.submitted2017-04-20
dc.identifier.citationMethods Mol Biol. 1 September 2016; 1465:159-73. doi: 10.1007/978-1-4939-4011-0_14. <a href="https://doi.org/10.1007/978-1-4939-4011-0_14">Link to article on publisher's site</a>
dc.identifier.issn1064-3745 (Linking)
dc.identifier.doi10.1007/978-1-4939-4011-0_14
dc.identifier.pmid27581147
dc.identifier.urihttp://hdl.handle.net/20.500.14038/36694
dc.description.abstractGenome-wide RNA interference (RNAi) screening in mammalian cells has proven to be a powerful tool for identifying new genes and molecular pathways relevant to many cellular processes and diseases. For example, screening for genes that, when inactivated, lead to resistance to cancer therapeutic drugs can reveal new mechanisms for how resistance develops and identify potential targetable strategies to overcome drug resistance. Here, we describe a detailed procedure for performing a high-throughput RNAi screen using a genome-wide human short hairpin RNA (shRNA) library for identifying tyrosine kinase inhibitor (TKI)-resistance genes in a human CML cell line model.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=27581147&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttps://doi.org/10.1007/978-1-4939-4011-0_14
dc.subjectCML
dc.subjectDrug resistance
dc.subjectGenome-wide
dc.subjectImatinib
dc.subjectRNAi
dc.subjectTyrosine kinase inhibitor
dc.subjectshRNA screen
dc.subjectBiochemistry
dc.subjectCell Biology
dc.subjectCellular and Molecular Physiology
dc.subjectGenomics
dc.subjectMolecular Biology
dc.titleHigh-Throughput Screening of Tyrosine Kinase Inhibitor Resistant Genes in CML
dc.typeJournal Article
dc.source.journaltitleMethods in molecular biology (Clifton, N.J.)
dc.source.volume1465
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/metnet_pubs/61
dc.identifier.contextkey10048083
html.description.abstract<p>Genome-wide RNA interference (RNAi) screening in mammalian cells has proven to be a powerful tool for identifying new genes and molecular pathways relevant to many cellular processes and diseases. For example, screening for genes that, when inactivated, lead to resistance to cancer therapeutic drugs can reveal new mechanisms for how resistance develops and identify potential targetable strategies to overcome drug resistance. Here, we describe a detailed procedure for performing a high-throughput RNAi screen using a genome-wide human short hairpin RNA (shRNA) library for identifying tyrosine kinase inhibitor (TKI)-resistance genes in a human CML cell line model.</p>
dc.identifier.submissionpathmetnet_pubs/61
dc.contributor.departmentUMass Metabolic Network
dc.contributor.departmentDepartment of Molecular, Cell and Cancer Biology
dc.source.pages159-73


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