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dc.contributor.authorBarutcu, Ahmet Rasim
dc.contributor.authorLian, Jane B.
dc.contributor.authorStein, Janet L.
dc.contributor.authorStein, Gary S.
dc.contributor.authorImbalzano, Anthony N.
dc.date2022-08-11T08:09:21.000
dc.date.accessioned2022-08-23T16:27:09Z
dc.date.available2022-08-23T16:27:09Z
dc.date.issued2017-03-04
dc.date.submitted2017-05-25
dc.identifier.citationNucleus. 2017 Mar 4;8(2):150-155. Epub 2017 Jan 6. <a href="https://doi.org/10.1080/19491034.2016.1276145">Link to article on publisher's site</a>
dc.identifier.issn1949-1034 (Linking)
dc.identifier.doi10.1080/19491034.2016.1276145
dc.identifier.pmid28060558
dc.identifier.urihttp://hdl.handle.net/20.500.14038/36717
dc.description.abstractThe eukaryotic genome is partitioned into topologically associating domains (TADs). Despite recent advances characterizing TADs and TAD boundaries, the organization of these structures is an important dimension of genome architecture and function that is not well understood. Recently, we demonstrated that knockdown of BRG1, an ATPase driving the chromatin remodeling activity of mammalian SWI/SNF enzymes, globally alters long-range genomic interactions and results in a reduction of TAD boundary strength. We provided evidence suggesting that this effect may be due to BRG1 affecting nucleosome occupancy around CTCF sites present at TAD boundaries. In this review, we elaborate on our findings and speculate that BRG1 may contribute to the regulation of the structural and functional properties of chromatin at TAD boundaries by affecting the function or the recruitment of CTCF and DNA topoisomerase complexes.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=28060558&dopt=Abstract">Link to Article in PubMed</a>
dc.rightsCopyright © 2017 The Author(s).
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBRG1
dc.subjectCTCF
dc.subjectHi-C
dc.subjectSWI/SNF
dc.subjectTADs
dc.subjecttopoisomerase
dc.subjecttopologically associated domains
dc.subjectBiochemistry
dc.subjectCell Biology
dc.subjectCellular and Molecular Physiology
dc.subjectMolecular Biology
dc.titleThe connection between BRG1, CTCF and topoisomerases at TAD boundaries
dc.typeJournal Article
dc.source.journaltitleNucleus (Austin, Tex.)
dc.source.volume8
dc.source.issue2
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1084&amp;context=metnet_pubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/metnet_pubs/85
dc.identifier.contextkey10212148
refterms.dateFOA2022-08-23T16:27:09Z
html.description.abstract<p>The eukaryotic genome is partitioned into topologically associating domains (TADs). Despite recent advances characterizing TADs and TAD boundaries, the organization of these structures is an important dimension of genome architecture and function that is not well understood. Recently, we demonstrated that knockdown of BRG1, an ATPase driving the chromatin remodeling activity of mammalian SWI/SNF enzymes, globally alters long-range genomic interactions and results in a reduction of TAD boundary strength. We provided evidence suggesting that this effect may be due to BRG1 affecting nucleosome occupancy around CTCF sites present at TAD boundaries. In this review, we elaborate on our findings and speculate that BRG1 may contribute to the regulation of the structural and functional properties of chromatin at TAD boundaries by affecting the function or the recruitment of CTCF and DNA topoisomerase complexes.</p>
dc.identifier.submissionpathmetnet_pubs/85
dc.contributor.departmentImbalzano Lab
dc.contributor.departmentUMass Metabolic Network
dc.contributor.departmentDepartment of Cell and Developmental Biology
dc.source.pages150-155


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