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dc.contributor.authorMaurizi, Giulia
dc.contributor.authorPoloni, Antonella
dc.contributor.authorCorvera, Silvia
dc.contributor.authorCinti, Saverio
dc.date2022-08-11T08:09:21.000
dc.date.accessioned2022-08-23T16:27:10Z
dc.date.available2022-08-23T16:27:10Z
dc.date.issued2017-10-01
dc.date.submitted2017-02-16
dc.identifier.citationJ Cell Physiol. 2017 Oct;232(10):2887-2899. doi: 10.1002/jcp.25743. Epub 2017 Apr 12. <a href="https://doi.org/10.1002/jcp.25743">Link to article on publisher's site</a>
dc.identifier.issn0021-9541 (Linking)
dc.identifier.doi10.1002/jcp.25743
dc.identifier.pmid27987321
dc.identifier.urihttp://hdl.handle.net/20.500.14038/36722
dc.description<p>Full list of authors omitted for brevity. For full list see article.</p> <p>This article is the cover image for the October 2017 issue of Journal of Cellular Physiology, PMID 28644916.</p>
dc.description.abstractWhite adipocytes are plastic cells able to reversibly transdifferentiate into brown adipocytes and into epithelial glandular cells under physiologic stimuli in vivo. These plastic properties could be used in future for regenerative medicine, but are incompletely explored in their details. Here, we focused on plastic properties of human mature adipocytes (MA) combining gene expression profile through microarray analysis with morphologic data obtained by electron and time lapse microscopy. Primary MA showed the classic morphology and gene expression profile of functional mature adipocytes. Notably, despite their committed status, MA expressed high levels of reprogramming genes. MA from ceiling cultures underwent transdifferentiation towards fibroblast-like cells with a well-differentiated morphology and maintaining stem cell gene signatures. The main morphologic aspect of the transdifferentiation process was the secretion of large lipid droplets and the development of organelles necessary for exocrine secretion further supported the liposecretion process. Of note, electron microscope findings suggesting liposecretion phenomena were found also in explants of human fat and rarely in vivo in fat biopsies from obese patients. In conclusion, both MA and post-liposecretion adipocytes show a well-differentiated phenotype with stem cell properties in line with the extraordinary plasticity of adipocytes in vivo.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=27987321&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttps://doi.org/10.1002/jcp.25743
dc.subjectBiochemistry
dc.subjectCell Biology
dc.subjectCellular and Molecular Physiology
dc.subjectMolecular Biology
dc.titleHuman white adipocytes convert into "rainbow" adipocytes in vitro
dc.typeJournal Article
dc.source.journaltitleJournal of cellular physiology
dc.source.volume232
dc.source.issue10
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/metnet_pubs/9
dc.identifier.contextkey9698863
html.description.abstract<p>White adipocytes are plastic cells able to reversibly transdifferentiate into brown adipocytes and into epithelial glandular cells under physiologic stimuli in vivo. These plastic properties could be used in future for regenerative medicine, but are incompletely explored in their details. Here, we focused on plastic properties of human mature adipocytes (MA) combining gene expression profile through microarray analysis with morphologic data obtained by electron and time lapse microscopy. Primary MA showed the classic morphology and gene expression profile of functional mature adipocytes. Notably, despite their committed status, MA expressed high levels of reprogramming genes. MA from ceiling cultures underwent transdifferentiation towards fibroblast-like cells with a well-differentiated morphology and maintaining stem cell gene signatures. The main morphologic aspect of the transdifferentiation process was the secretion of large lipid droplets and the development of organelles necessary for exocrine secretion further supported the liposecretion process. Of note, electron microscope findings suggesting liposecretion phenomena were found also in explants of human fat and rarely in vivo in fat biopsies from obese patients. In conclusion, both MA and post-liposecretion adipocytes show a well-differentiated phenotype with stem cell properties in line with the extraordinary plasticity of adipocytes in vivo.</p>
dc.identifier.submissionpathmetnet_pubs/9
dc.contributor.departmentUMass Metabolic Network
dc.contributor.departmentProgram in Molecular Medicine
dc.source.pages2887-2899


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