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A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae

dc.contributor.authorAkerley, Brian J.
dc.contributor.authorRubin, Eric J.
dc.contributor.authorNovik, Veronica N.
dc.contributor.authorAmaya, Kensey
dc.contributor.authorJudson, Nicholas
dc.contributor.authorMekalanos, John J.
dc.date2022-08-11T08:09:25.000
dc.date.accessioned2022-08-23T16:29:53Z
dc.date.available2022-08-23T16:29:53Z
dc.date.issued2002-01-24
dc.date.submitted2010-02-01
dc.identifier.citationProc Natl Acad Sci U S A. 2002 Jan 22;99(2):966-71. <a href="http://dx.doi.org/10.1073/pnas.012602299">Link to article on publisher's site</a>
dc.identifier.issn0027-8424 (Print)
dc.identifier.doi10.1073/pnas.012602299
dc.identifier.urihttp://hdl.handle.net/20.500.14038/37351
dc.description.abstractA high-density transposon mutagenesis strategy was applied to the Haemophilus influenzae genome to identify genes required for growth or viability. This analysis detected putative essential roles for the products of 259 ORFs of unknown function. Comparisons between complete genomes defined a subset of these proteins in H. influenzae having homologs in Mycobacterium tuberculosis that are absent in Saccharomyces cerevisiae, a distribution pattern that favors their use in development of antimicrobial therapeutics. Three genes within this set are essential for viability in other bacteria. Interfacing the set of essential gene products in H. influenzae with the distribution of homologs in other microorganisms can detect components of unrecognized cellular pathways essential in diverse bacteria. This genome-scale phenotypic analysis identifies potential roles for a large set of genes of unknown function.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=11805338&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1073/pnas.012602299
dc.subjectBase Sequence
dc.subjectChromosome Mapping
dc.subjectChromosomes, Bacterial
dc.subjectGenes, Bacterial
dc.subject*Genome, Bacterial
dc.subjectHaemophilus influenzae
dc.subjectMutagenesis, Insertional
dc.subjectOpen Reading Frames
dc.subjectPhenotype
dc.subjectPlasmids
dc.subjectMicrobiology
dc.subjectMolecular Genetics
dc.titleA genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae
dc.typeJournal Article
dc.source.journaltitleProceedings of the National Academy of Sciences of the United States of America
dc.source.volume99
dc.source.issue2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/mgm_pp/16
dc.identifier.contextkey1127075
html.description.abstract<p>A high-density transposon mutagenesis strategy was applied to the Haemophilus influenzae genome to identify genes required for growth or viability. This analysis detected putative essential roles for the products of 259 ORFs of unknown function. Comparisons between complete genomes defined a subset of these proteins in H. influenzae having homologs in Mycobacterium tuberculosis that are absent in Saccharomyces cerevisiae, a distribution pattern that favors their use in development of antimicrobial therapeutics. Three genes within this set are essential for viability in other bacteria. Interfacing the set of essential gene products in H. influenzae with the distribution of homologs in other microorganisms can detect components of unrecognized cellular pathways essential in diverse bacteria. This genome-scale phenotypic analysis identifies potential roles for a large set of genes of unknown function.</p>
dc.identifier.submissionpathmgm_pp/16
dc.contributor.departmentDepartment of Molecular Genetics and Microbiology
dc.source.pages966-71


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