Show simple item record

dc.contributor.authorPollack, Shimon
dc.contributor.authorLedbetter, Jeffrey A.
dc.contributor.authorKatz, Rina
dc.contributor.authorWilliams, Katherine
dc.contributor.authorAkerley, Brian J.
dc.contributor.authorFranklin, Kymberly
dc.contributor.authorSchieven, Gary L.
dc.contributor.authorNel, Andre E.
dc.date2022-08-11T08:09:25.000
dc.date.accessioned2022-08-23T16:29:54Z
dc.date.available2022-08-23T16:29:54Z
dc.date.issued1991-06-01
dc.date.submitted2010-02-01
dc.identifier.citationBiochem J. 1991 Jun 1;276 ( Pt 2):481-5.
dc.identifier.issn0264-6021 (Print)
dc.identifier.urihttp://hdl.handle.net/20.500.14038/37355
dc.description.abstractLigation of CD3/TCR on T-cells induces transient activation of lymphoid MAP-2 kinase (MAP-2K), a 43 kDa serine kinase which itself is a substrate of an unidentified tyrosine kinase (pp43). The reversibility of the MAP-2K response agrees with removal of tyrosine phosphates from pp43. Since both activity as well as tyrosine phosphorylation of MAP-2K could be prolonged by Na3VO4, a phosphotyrosine phosphatase inhibitor, we studied the effect of the common CD45 isoform, which is a member of the CD45 phosphatase family, on MAP-2K activity in vivo and in vitro. We demonstrate the ability of purified CD45 phosphatase to remove tyrosine phosphates from partially purified lymphoid MAP-2K. Utilizing the approach of heterologous receptor aggregation, we also showed that CD45 could inhibit the induction of MAP-2K activity in intact Jurkat cells during CD3 or CD3 + CD4 stimulation. We therefore suggest that this phosphatase may control the activity of lymphoid MAP-2K in vivo.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=1710891&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1151116/?tool=pubmed
dc.subjectAntigens, CD
dc.subjectAntigens, CD3
dc.subjectAntigens, CD45
dc.subjectAntigens, Differentiation
dc.subjectAntigens, Differentiation, T-Lymphocyte
dc.subjectCalcium
dc.subjectCalcium-Calmodulin-Dependent Protein Kinases
dc.subjectCell Line
dc.subjectEnzyme Activation
dc.subjectHistocompatibility Antigens
dc.subjectHumans
dc.subjectKinetics
dc.subjectMicrotubules
dc.subjectPhosphoprotein Phosphatases
dc.subjectPhosphorylation
dc.subjectPhosphotyrosine
dc.subjectProtein Kinases
dc.subjectReceptors, Antigen, T-Cell
dc.subjectT-Lymphocytes
dc.subjectTyrosine
dc.subjectMicrobiology
dc.subjectMolecular Genetics
dc.titleEvidence for involvement of glycoprotein-CD45 phosphatase in reversing glycoprotein-CD3-induced microtubule-associated protein-2 kinase activity in Jurkat T-cells
dc.typeJournal Article
dc.source.journaltitleThe Biochemical journal
dc.source.volume276 ( Pt 2)
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/mgm_pp/2
dc.identifier.contextkey1127061
html.description.abstract<p>Ligation of CD3/TCR on T-cells induces transient activation of lymphoid MAP-2 kinase (MAP-2K), a 43 kDa serine kinase which itself is a substrate of an unidentified tyrosine kinase (pp43). The reversibility of the MAP-2K response agrees with removal of tyrosine phosphates from pp43. Since both activity as well as tyrosine phosphorylation of MAP-2K could be prolonged by Na3VO4, a phosphotyrosine phosphatase inhibitor, we studied the effect of the common CD45 isoform, which is a member of the CD45 phosphatase family, on MAP-2K activity in vivo and in vitro. We demonstrate the ability of purified CD45 phosphatase to remove tyrosine phosphates from partially purified lymphoid MAP-2K. Utilizing the approach of heterologous receptor aggregation, we also showed that CD45 could inhibit the induction of MAP-2K activity in intact Jurkat cells during CD3 or CD3 + CD4 stimulation. We therefore suggest that this phosphatase may control the activity of lymphoid MAP-2K in vivo.</p>
dc.identifier.submissionpathmgm_pp/2
dc.contributor.departmentDepartment of Molecular Genetics and Microbiology
dc.source.pages481-5


This item appears in the following Collection(s)

Show simple item record