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dc.contributor.authorDong, Shuyun
dc.contributor.authorJacobson, Allan
dc.contributor.authorHe, Feng
dc.date2022-08-11T08:09:25.000
dc.date.accessioned2022-08-23T16:29:57Z
dc.date.available2022-08-23T16:29:57Z
dc.date.issued2010-04-27
dc.date.submitted2010-04-28
dc.identifier.citationDong S, Jacobson A, He F (2010) Degradation of YRA1 Pre-mRNA in the Cytoplasm Requires Translational Repression, Multiple Modular Intronic Elements, Edc3p, and Mex67p. PLoS Biol 8(4): e1000360. <a href="http://dx.doi.org/10.1371/journal.pbio.1000360">Link to article on publisher's website</a>
dc.identifier.doi10.1371/journal.pbio.1000360
dc.identifier.urihttp://hdl.handle.net/20.500.14038/37364
dc.description.abstractIntron-containing pre-mRNAs are normally retained and processed in the nucleus but are sometimes exported to the cytoplasm and degraded by the nonsense-mediated mRNA decay (NMD) pathway as a consequence of their inclusion of intronic in-frame termination codons. When shunted to the cytoplasm by autoregulated nuclear export, the intron-containing yeast YRA1 pre-mRNA evades NMD and is targeted by a cytoplasmic decay pathway mediated by the decapping activator Edc3p. Here, we have elucidated this transcript-specific decay mechanism, showing that Edc3p-mediated YRA1 pre-mRNA degradation occurs independently of translation and is controlled through five structurally distinct but functionally interdependent modular elements in the YRA1 intron. Two of these elements target the pre-mRNA as an Edc3p substrate and the other three mediate transcript-specific translational repression. Translational repression of YRA1 pre-mRNA also requires the heterodimeric Mex67p/Mtr2p general mRNA export receptor, but not Edc3p, and serves to enhance Edc3p substrate specificity by inhibiting the susceptibility of this pre-mRNA to NMD. Collectively, our data indicate that YRA1 pre-mRNA degradation is a highly regulated process that proceeds through translational repression, substrate recognition by Edc3p, recruitment of the Dcp1p/Dcp2p decapping enzyme, and activation of decapping.
dc.language.isoen_US
dc.subjectRNA, Messenger
dc.subjectRNA Stability
dc.subjectProtein Biosynthesis
dc.subjectIntrons
dc.subjectNuclear Proteins
dc.subjectRNA-Binding Proteins
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectRNA Cap-Binding Proteins
dc.subjectRNA Caps
dc.subjectMicrobiology
dc.subjectMolecular Genetics
dc.titleDegradation of YRA1 Pre-mRNA in the Cytoplasm Requires Translational Repression, Multiple Modular Intronic Elements, Edc3p, and Mex67p
dc.typeJournal Article
dc.source.journaltitlePLoS Biology
dc.source.volume8
dc.source.issue4
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1027&amp;context=mgm_pp&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/mgm_pp/28
dc.identifier.contextkey1290085
refterms.dateFOA2022-08-23T16:29:58Z
html.description.abstract<p>Intron-containing pre-mRNAs are normally retained and processed in the nucleus but are sometimes exported to the cytoplasm and degraded by the nonsense-mediated mRNA decay (NMD) pathway as a consequence of their inclusion of intronic in-frame termination codons. When shunted to the cytoplasm by autoregulated nuclear export, the intron-containing yeast YRA1 pre-mRNA evades NMD and is targeted by a cytoplasmic decay pathway mediated by the decapping activator Edc3p. Here, we have elucidated this transcript-specific decay mechanism, showing that Edc3p-mediated YRA1 pre-mRNA degradation occurs independently of translation and is controlled through five structurally distinct but functionally interdependent modular elements in the YRA1 intron. Two of these elements target the pre-mRNA as an Edc3p substrate and the other three mediate transcript-specific translational repression. Translational repression of YRA1 pre-mRNA also requires the heterodimeric Mex67p/Mtr2p general mRNA export receptor, but not Edc3p, and serves to enhance Edc3p substrate specificity by inhibiting the susceptibility of this pre-mRNA to NMD. Collectively, our data indicate that YRA1 pre-mRNA degradation is a highly regulated process that proceeds through translational repression, substrate recognition by Edc3p, recruitment of the Dcp1p/Dcp2p decapping enzyme, and activation of decapping.</p>
dc.identifier.submissionpathmgm_pp/28
dc.contributor.departmentDepartment of Molecular Genetics and Microbiology
dc.source.pagese1000360


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