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Reproducibility and reliability of middle cerebral artery occlusion using a silicone-coated suture (Koizumi) in rats

dc.contributor.authorTakano, Kentaro
dc.contributor.authorTatlisumak, Turgut
dc.contributor.authorBergmann, Andreas G.
dc.contributor.authorGibson, Daniel G. III
dc.contributor.authorFisher, Marc
dc.date2022-08-11T08:09:26.000
dc.date.accessioned2022-08-23T16:31:06Z
dc.date.available2022-08-23T16:31:06Z
dc.date.issued1997-12-09
dc.date.submitted2008-04-17
dc.identifier.citationJ Neurol Sci. 1997 Dec 9;153(1):8-11.
dc.identifier.issn0022-510X (Print)
dc.identifier.pmid9455971
dc.identifier.urihttp://hdl.handle.net/20.500.14038/37567
dc.description.abstractThe best technical approach to rat middle cerebral artery occlusion (MCAO) using a nylon-monofilament suture remains unsettled, regarding the usefulness of coated or uncoated sutures. Three investigators with different degrees of experience: A, well skilled; B, 2 years of experience; C, a novice with 6 months of experience, each subjected 10 Sprague-Dawley rats to permanent MCAO using low-viscosity silicone-coated sutures with a mean diameter 0.468+/-0.013 mm (mean+/-S.D.) at the tip and 0.361+/-0.013 mm in the body. Post-mortem corrected infarct size 24 h after MCAO was similar among the three investigators: A, 204.7+/-33.2 mm3; B, 212.6+/-42.8, and C, 195.9+/-44.4. The coefficient of variation was 16.2% to 22.7%, and 19.4% for the three investigators. This study suggests that experimental stroke with silicone-coated sutures (Koizumi's method) provides good reproducibility and reliability, among investigators of varying experience.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9455971&dopt=Abstract ">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/S0022-510X(97)00184-6
dc.subjectAnimals
dc.subjectBrain Ischemia
dc.subjectCerebral Arteries
dc.subjectDisease Models, Animal
dc.subjectMale
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectSilicones
dc.subject*Sutures
dc.subjectNervous System Diseases
dc.subjectNeurology
dc.titleReproducibility and reliability of middle cerebral artery occlusion using a silicone-coated suture (Koizumi) in rats
dc.typeJournal Article
dc.source.journaltitleJournal of the neurological sciences
dc.source.volume153
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/neuro_pp/106
dc.identifier.contextkey492212
html.description.abstract<p>The best technical approach to rat middle cerebral artery occlusion (MCAO) using a nylon-monofilament suture remains unsettled, regarding the usefulness of coated or uncoated sutures. Three investigators with different degrees of experience: A, well skilled; B, 2 years of experience; C, a novice with 6 months of experience, each subjected 10 Sprague-Dawley rats to permanent MCAO using low-viscosity silicone-coated sutures with a mean diameter 0.468+/-0.013 mm (mean+/-S.D.) at the tip and 0.361+/-0.013 mm in the body. Post-mortem corrected infarct size 24 h after MCAO was similar among the three investigators: A, 204.7+/-33.2 mm3; B, 212.6+/-42.8, and C, 195.9+/-44.4. The coefficient of variation was 16.2% to 22.7%, and 19.4% for the three investigators. This study suggests that experimental stroke with silicone-coated sutures (Koizumi's method) provides good reproducibility and reliability, among investigators of varying experience.</p>
dc.identifier.submissionpathneuro_pp/106
dc.contributor.departmentDepartment of Neurology
dc.source.pages8-11


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