Effects of intravenous dimethyl sulfoxide on ischemia evolution in a rat permanent occlusion model
AuthorsBardutzky, Juergen F.
Bouley, James P.
Duong, Timothy Q.
UMass Chan AffiliationsDepartment of Neurology
Diffusion Magnetic Resonance Imaging
Infarction, Middle Cerebral Artery
Rats, Inbred WKY
Nervous System Diseases
MetadataShow full item record
AbstractDimethyl sulfoxide (DMSO) has a variety of biological actions that suggest efficacy as a neuroprotectant. We (1) tested the neuroprotective potential of DMSO at different time windows on infarct size using 2,3,5-triphenyltetrazolium staining and (2) investigated the effects of DMSO on ischemia evolution using quantitative diffusion and perfusion imaging in a permanent middle cerebral artery occlusion (MCAO) model in rats. In experiment 1, DMSO treatment (1.5 g/kg intravenously over 3 h) reduced infarct volume 24 h after MCAO by 65% (P<0.00001) when initiated 20 h before MCAO, by 44% (P=0.0006) when initiated 1 h after MCAO, and by 17% (P=0.11) when started 2 h after MCAO. Significant infarct reduction was also observed after a 3-day survival in animals treated 1 h after MCAO (P=0.005). In experiment 2, treatment was initiated 1 h after MCAO and maps for cerebral blood flow (CBF) and apparent diffusion coefficient (ADC) were acquired before treatment and then every 30 mins up to 4 h. Cerebral blood flow characteristics and CBF-derived lesion volumes did not differ between treated and untreated animals, whereas the ADC-derived lesion volume essentially stopped progressing during DMSO treatment, resulting in a persistent diffusion/perfusion mismatch. This effect was mainly observed in the cortex. Our data suggest that DMSO represents an interesting candidate for acute stroke treatment.
SourceJ Cereb Blood Flow Metab. 2005 Aug;25(8):968-77. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/37631
Related ResourcesLink to article in PubMed