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dc.contributor.authorGerriets, Tibo
dc.contributor.authorStolz, Erwin
dc.contributor.authorWalberer, Maureen
dc.contributor.authorMuller, Clemens
dc.contributor.authorKluge, Alexander
dc.contributor.authorKaps, Manfred
dc.contributor.authorFisher, Marc
dc.contributor.authorBachmann, Georg
dc.date2022-08-11T08:09:27.000
dc.date.accessioned2022-08-23T16:31:24Z
dc.date.available2022-08-23T16:31:24Z
dc.date.issued2004-03-12
dc.date.submitted2008-04-28
dc.identifier.citationBrain Res Brain Res Protoc. 2004 Feb;12(3):137-43. <a href="http://dx.doi.org/10.1016/j.brainresprot.2003.08.006">Link to article on publisher's site</a>
dc.identifier.issn1385-299X (Print)
dc.identifier.doi10.1016/j.brainresprot.2003.08.006
dc.identifier.pmid15013464
dc.identifier.urihttp://hdl.handle.net/20.500.14038/37635
dc.description.abstractMagnetic resonance imaging (MRI) provides insights into the dynamics of focal cerebral ischemia. Usually, experimental stroke is induced outside the magnet bore, preventing investigators from acquiring pre-ischemic images for later pixel-by-pixel comparisons and from studying the earliest changes in the hyperacute phase of ischemia. Herein, we introduce a new and easy to apply in-bore occlusion protocol based on the intraarterial embolization of ceramic macrospheres. PE-50 tubing, filled with saline and six macrospheres (0.315-0.355 mm in diameter), was placed into the internal carotid artery (ICA) of anesthetized Sprague-Dawley rats. The animals were transferred into an MRI scanner (7.0 T) and baseline diffusion-weighted imaging (DWI) and T2-imaging was performed. Then the macrospheres were injected into the internal artery to occlude the MCA. Post-ischemic DWI and T2-imaging was started immediately thereafter. The apparent diffusion coefficient (ADC) (a marker for cytotoxic brain edema) and T2-relaxation time (a marker for vasogenic brain edema) were determined in the ischemic lesions and compared to the unaffected hemisphere. ADC significantly declined within the first 5-10 min after stroke onset. T2-relaxation time increased as early as at the first T2-imaging time-point (20-35 min after embolization). After 150 min of ischemia, the lesions covered 18.0 +/- 7.4% of the hemispheres. The model failed in one out of nine animals (11%). This model allows MR-imaging from the initial minutes after permanent middle cerebral artery (MCA) occlusion. It does not permit reperfusion. This technique might provide information about the pathophysiological processes in the hyperacute phase of stroke.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15013464&dopt=Abstract ">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/j.brainresprot.2003.08.006
dc.subjectAcute Disease
dc.subjectAnimals
dc.subjectBrain
dc.subjectBrain Edema
dc.subject*Ceramics
dc.subjectCerebral Infarction
dc.subjectCerebrovascular Circulation
dc.subjectDisease Models, Animal
dc.subjectDisease Progression
dc.subjectInfarction, Middle Cerebral Artery
dc.subjectMagnetic Resonance Imaging
dc.subject*Microspheres
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectReaction Time
dc.subjectVascular Surgical Procedures
dc.subjectNeurology
dc.subjectRadiology
dc.titleMiddle cerebral artery occlusion during MR-imaging: investigation of the hyperacute phase of stroke using a new in-bore occlusion model in rats
dc.typeJournal Article
dc.source.journaltitleBrain research. Brain research protocols
dc.source.volume12
dc.source.issue3
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/neuro_pp/178
dc.identifier.contextkey499360
html.description.abstract<p>Magnetic resonance imaging (MRI) provides insights into the dynamics of focal cerebral ischemia. Usually, experimental stroke is induced outside the magnet bore, preventing investigators from acquiring pre-ischemic images for later pixel-by-pixel comparisons and from studying the earliest changes in the hyperacute phase of ischemia. Herein, we introduce a new and easy to apply in-bore occlusion protocol based on the intraarterial embolization of ceramic macrospheres. PE-50 tubing, filled with saline and six macrospheres (0.315-0.355 mm in diameter), was placed into the internal carotid artery (ICA) of anesthetized Sprague-Dawley rats. The animals were transferred into an MRI scanner (7.0 T) and baseline diffusion-weighted imaging (DWI) and T2-imaging was performed. Then the macrospheres were injected into the internal artery to occlude the MCA. Post-ischemic DWI and T2-imaging was started immediately thereafter. The apparent diffusion coefficient (ADC) (a marker for cytotoxic brain edema) and T2-relaxation time (a marker for vasogenic brain edema) were determined in the ischemic lesions and compared to the unaffected hemisphere. ADC significantly declined within the first 5-10 min after stroke onset. T2-relaxation time increased as early as at the first T2-imaging time-point (20-35 min after embolization). After 150 min of ischemia, the lesions covered 18.0 +/- 7.4% of the hemispheres. The model failed in one out of nine animals (11%). This model allows MR-imaging from the initial minutes after permanent middle cerebral artery (MCA) occlusion. It does not permit reperfusion. This technique might provide information about the pathophysiological processes in the hyperacute phase of stroke.</p>
dc.identifier.submissionpathneuro_pp/178
dc.contributor.departmentDepartment of Neurology
dc.source.pages137-43


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