Myelination and long diffusion times alter diffusion-tensor-imaging contrast in myelin-deficient shiverer mice
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Authors
Nair, GovindTanahashi, Yusuke
Low, Hoi Pang
Billings-Gagliardi, Susan
Schwartz, William J.
Duong, Timothy Q.
Document Type
Journal ArticlePublication Date
2005-07-19Keywords
AlgorithmsAnimals
Animals, Newborn
Anisotropy
Corpus Callosum
Demyelinating Diseases
*Diffusion Magnetic Resonance Imaging
Image Processing, Computer-Assisted
Immunohistochemistry
Mice
Mice, Neurologic Mutants
Myelin Sheath
Neural Pathways
Neurons
Stem Cell Transplantation
Cell Biology
Molecular and Cellular Neuroscience
Radiology
Metadata
Show full item recordAbstract
Diffusion tensor imaging (DTI) using variable diffusion times (t(diff)) was performed to investigate wild-type (wt) mice, myelin-deficient shiverer (shi) mutant mice and shi mice transplanted with wt neural precursor cells that differentiate and function as oligodendrocytes. At t(diff) = 30 ms, the diffusion anisotropy "volume ratio" (VR), diffusion perpendicular to the fibers (lambda( perpendicular)), and mean apparent diffusion coefficient () of the corpus callosum of shi mice were significantly higher than those of wt mice by 12 +/- 2%, 13 +/- 2%, and 10 +/- 1%, respectively; fractional anisotropy (FA) and relative anisotropy (RA) were lower by 10 +/- 1% and 11 +/- 3%, respectively. Diffusion parallel to the fibers (lambda(//)) was not statistically different between shi and wt mice. Normalized T(2)-weighted signal intensities showed obvious differences (27 +/- 4%) between wt and shi mice in the corpus callosum but surprisingly did not detect transplant-derived myelination. In contrast, diffusion anisotropy maps detected transplant-derived myelination in the corpus callosum and its spatial distribution was consistent with the donor-derived myelination determined by immunohistochemical staining. Anisotropy indices (except lambda(//)) in the corpus callosum showed strong t(diff) dependence (30-280 ms), and the differences in lambda( perpendicular) and VR between wt and shi mice became significantly larger at longer t(diff), indicative of improved DTI sensitivity at long t(diff). In contrast, anisotropy indices in the hippocampus showed very weak t(diff) dependence and were not significantly different between wt and shi mice across different t(diff). This study provides insights into the biological signal sources and measurement parameters influencing DTI contrast, which could lead to developing more sensitive techniques for detection of demyelinating diseases.Source
Neuroimage. 2005 Oct 15;28(1):165-74. Epub 2005 Jul 14. Link to article on publisher's siteDOI
10.1016/j.neuroimage.2005.05.049Permanent Link to this Item
http://hdl.handle.net/20.500.14038/37644PubMed ID
16023870Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/j.neuroimage.2005.05.049