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dc.contributor.authorKelsch, Wolfgang
dc.contributor.authorStolfi, Alberto
dc.contributor.authorLois, Carlos
dc.date2022-08-11T08:09:29.000
dc.date.accessioned2022-08-23T16:32:18Z
dc.date.available2022-08-23T16:32:18Z
dc.date.issued2012-06-07
dc.date.submitted2012-09-07
dc.identifier.citationKelsch W, Stolfi A, Lois C (2012) Genetic Labeling of Neuronal Subsets through Enhancer Trapping in Mice. PLoS ONE 7(6): e38593. doi:10.1371/journal.pone.0038593. <a href="http://dx.doi.org/10.1371/journal.pone.0038593" target="_blank">Link to article on publisher's site</a>
dc.identifier.issn1932-6203 (Linking)
dc.identifier.doi10.1371/journal.pone.0038593
dc.identifier.pmid22685588
dc.identifier.urihttp://hdl.handle.net/20.500.14038/37854
dc.description.abstractThe ability to label, visualize, and manipulate subsets of neurons is critical for elucidating the structure and function of individual cell types in the brain. Enhancer trapping has proved extremely useful for the genetic manipulation of selective cell types in Drosophila. We have developed an enhancer trap strategy in mammals by generating transgenic mice with lentiviral vectors carrying single-copy enhancer-detector probes encoding either the marker gene lacZ or Cre recombinase. This transgenic strategy allowed us to genetically identify a wide variety of neuronal subpopulations in distinct brain regions. Enhancer detection by lentiviral transgenesis could thus provide a complementary method for generating transgenic mouse libraries for the genetic labeling and manipulation of neuronal subsets.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=22685588&dopt=Abstract">Link to Article in PubMed</a>
dc.rightsCopyright: © 2012 Kelsch et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.subjectEnhancer Elements, Genetic
dc.subjectNeurons
dc.subjectNeuroscience and Neurobiology
dc.titleGenetic labeling of neuronal subsets through enhancer trapping in mice
dc.typeJournal Article
dc.source.journaltitlePloS one
dc.source.volume7
dc.source.issue6
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1119&amp;context=neurobiology_pp&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/neurobiology_pp/120
dc.identifier.contextkey3302517
refterms.dateFOA2022-08-23T16:32:18Z
html.description.abstract<p>The ability to label, visualize, and manipulate subsets of neurons is critical for elucidating the structure and function of individual cell types in the brain. Enhancer trapping has proved extremely useful for the genetic manipulation of selective cell types in Drosophila. We have developed an enhancer trap strategy in mammals by generating transgenic mice with lentiviral vectors carrying single-copy enhancer-detector probes encoding either the marker gene lacZ or Cre recombinase. This transgenic strategy allowed us to genetically identify a wide variety of neuronal subpopulations in distinct brain regions. Enhancer detection by lentiviral transgenesis could thus provide a complementary method for generating transgenic mouse libraries for the genetic labeling and manipulation of neuronal subsets.</p>
dc.identifier.submissionpathneurobiology_pp/120
dc.contributor.departmentLois Lab
dc.contributor.departmentNeurobiology
dc.source.pagese38593


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