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dc.contributor.authorSchumacher, Jennifer A.
dc.contributor.authorHsieh, Yi-Wen
dc.contributor.authorChen, Shiuhwei
dc.contributor.authorPirri, Jennifer K.
dc.contributor.authorAlkema, Mark J
dc.contributor.authorLi, Wen-hong
dc.contributor.authorChang, Chieh
dc.contributor.authorChuang, Chiou-Fen
dc.date2022-08-11T08:09:29.000
dc.date.accessioned2022-08-23T16:32:20Z
dc.date.available2022-08-23T16:32:20Z
dc.date.issued2012-11-01
dc.date.submitted2013-01-23
dc.identifier.citationDevelopment. 2012 Nov;139(22):4191-201. doi: 10.1242/dev.083428. <a href="http://dx.doi.org/10.1242/dev.083428">Link to article on publisher's site</a>
dc.identifier.issn0950-1991 (Linking)
dc.identifier.doi10.1242/dev.083428
dc.identifier.pmid23093425
dc.identifier.urihttp://hdl.handle.net/20.500.14038/37863
dc.description<p>Co-author Jennifer Pirri is a doctoral student in the Neuroscience program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.</p>
dc.description.abstractThe C. elegans left and right AWC olfactory neurons specify asymmetric subtypes, one default AWC(OFF) and one induced AWC(ON), through a stochastic, coordinated cell signaling event. Intercellular communication between AWCs and non-AWC neurons via a NSY-5 gap junction network coordinates AWC asymmetry. However, the nature of intercellular signaling across the network and how individual non-AWC cells in the network influence AWC asymmetry is not known. Here, we demonstrate that intercellular calcium signaling through the NSY-5 gap junction neural network coordinates a precise 1AWC(ON)/1AWC(OFF) decision. We show that NSY-5 gap junctions in C. elegans cells mediate small molecule passage. We expressed vertebrate calcium-buffer proteins in groups of cells in the network to reduce intracellular calcium levels, thereby disrupting intercellular communication. We find that calcium in non-AWC cells of the network promotes the AWC(ON) fate, in contrast to the autonomous role of calcium in AWCs to promote the AWC(OFF) fate. In addition, calcium in specific non-AWCs promotes AWC(ON) side biases through NSY-5 gap junctions. Our results suggest a novel model in which calcium has dual roles within the NSY-5 network: autonomously promoting AWC(OFF) and non-autonomously promoting AWC(ON).
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=23093425&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1242/dev.083428
dc.rightsPublisher PDF posted as allowed by the publisher's author rights policy at http://dev.biologists.org/content/rights-permissions.
dc.subjectCaenorhabditis elegans
dc.subjectCaenorhabditis elegans Proteins
dc.subjectCalcium Signaling
dc.subjectConnexins
dc.subjectGap Junctions
dc.subjectNeurons
dc.subjectOlfactory Receptor Neurons
dc.subjectGap junctions
dc.subjectCalcium signaling
dc.subjectStochastic left-right neuronal asymmetry
dc.subjectC. elegans
dc.subjectDevelopmental Neuroscience
dc.subjectMolecular and Cellular Neuroscience
dc.titleIntercellular calcium signaling in a gap junction-coupled cell network establishes asymmetric neuronal fates in C. elegans
dc.typeJournal Article
dc.source.journaltitleDevelopment (Cambridge, England)
dc.source.volume139
dc.source.issue22
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1133&amp;context=neurobiology_pp&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/neurobiology_pp/134
dc.identifier.contextkey3608528
refterms.dateFOA2022-08-23T16:32:20Z
html.description.abstract<p>The C. elegans left and right AWC olfactory neurons specify asymmetric subtypes, one default AWC(OFF) and one induced AWC(ON), through a stochastic, coordinated cell signaling event. Intercellular communication between AWCs and non-AWC neurons via a NSY-5 gap junction network coordinates AWC asymmetry. However, the nature of intercellular signaling across the network and how individual non-AWC cells in the network influence AWC asymmetry is not known. Here, we demonstrate that intercellular calcium signaling through the NSY-5 gap junction neural network coordinates a precise 1AWC(ON)/1AWC(OFF) decision. We show that NSY-5 gap junctions in C. elegans cells mediate small molecule passage. We expressed vertebrate calcium-buffer proteins in groups of cells in the network to reduce intracellular calcium levels, thereby disrupting intercellular communication. We find that calcium in non-AWC cells of the network promotes the AWC(ON) fate, in contrast to the autonomous role of calcium in AWCs to promote the AWC(OFF) fate. In addition, calcium in specific non-AWCs promotes AWC(ON) side biases through NSY-5 gap junctions. Our results suggest a novel model in which calcium has dual roles within the NSY-5 network: autonomously promoting AWC(OFF) and non-autonomously promoting AWC(ON).</p>
dc.identifier.submissionpathneurobiology_pp/134
dc.contributor.departmentGraduate School of Biomedical Sciences, Neuroscience Program
dc.contributor.departmentAlkema Lab
dc.contributor.departmentNeurobiology
dc.source.pages4191-201
dc.contributor.studentJennifer K. (Pirri) Ingemi
dc.description.thesisprogramNeuroscience


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