Student Authors
Preethi RamachandranAcademic Program
NeuroscienceUMass Chan Affiliations
Graduate School of Biomedical Sciences, Neuroscience ProgramBudnik Lab
Neurobiology
Document Type
Journal ArticlePublication Date
2010-08-01Keywords
AnimalsDissection
Drosophila melanogaster
Larva
Magnetics
Muscles
Developmental Neuroscience
Molecular and Cellular Neuroscience
Metadata
Show full item recordAbstract
Over the last two decades, the Drosophila larval neuromuscular junction has gained immense popularity as a model system for the study of synaptic development, function, and plasticity. With this model, it is easy to visualize synapses and manipulate the system genetically with a high degree of temporal and spatial control, which makes it ideal for resolving problems in synaptic physiology and development. A number of different techniques have been used to dissect third-instar larval preparations to expose the body-wall muscles. Here, we describe a procedure that uses magnetic chambers and pins to allow for fine control in spreading the larval body wall.Source
Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5469. doi: 10.1101/pdb.prot5469.DOI
10.1101/pdb.prot5469Permanent Link to this Item
http://hdl.handle.net/20.500.14038/37869PubMed ID
20679378Notes
First author Preethi Ramachandran is a doctoral student in the Neuroscience Program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.
Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1101/pdb.prot5469