Protein Gq modulates termination of phototransduction and prevents retinal degeneration
dc.contributor.author | Hu, Wen | |
dc.contributor.author | Wan, Didi | |
dc.contributor.author | Yu, Xiaoming | |
dc.contributor.author | Cao, Jinguo | |
dc.contributor.author | Guo, Peiyi | |
dc.contributor.author | Li, Hong-Sheng | |
dc.contributor.author | Han, Junhai | |
dc.date | 2022-08-11T08:09:29.000 | |
dc.date.accessioned | 2022-08-23T16:32:22Z | |
dc.date.available | 2022-08-23T16:32:22Z | |
dc.date.issued | 2012-04-20 | |
dc.date.submitted | 2013-01-23 | |
dc.identifier.citation | J Biol Chem. 2012 Apr 20;287(17):13911-8. doi: 10.1074/jbc.M112.339895. <a href="http://dx.doi.org/10.1074/jbc.M112.339895">Link to article on publisher's site</a> | |
dc.identifier.issn | 0021-9258 (Linking) | |
dc.identifier.doi | 10.1074/jbc.M112.339895 | |
dc.identifier.pmid | 22389492 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/37872 | |
dc.description | <p>Co-author Peiyi Guo is a doctoral student in the Neuroscience Program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.</p> | |
dc.description.abstract | Appropriate termination of the phototransduction cascade is critical for photoreceptors to achieve high temporal resolution and to prevent excessive Ca(2+)-induced cell toxicity. Using a genetic screen to identify defective photoresponse mutants in Drosophila, we isolated and identified a novel Galpha(q) mutant allele, which has defects in both activation and deactivation. We revealed that G(q) modulates the termination of the light response and that metarhodopsin/G(q) interaction affects subsequent arrestin-rhodopsin (Arr2-Rh1) binding, which mediates the deactivation of metarhodopsin. We further showed that the Galpha(q) mutant undergoes light-dependent retinal degeneration, which is due to the slow accumulation of stable Arr2-Rh1 complexes. Our study revealed the roles of G(q) in mediating photoresponse termination and in preventing retinal degeneration. This pathway may represent a general rapid feedback regulation of G protein-coupled receptor signaling. | |
dc.language.iso | en_US | |
dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=22389492&dopt=Abstract">Link to Article in PubMed</a> | |
dc.relation.url | http://dx.doi.org/10.1074/jbc.M112.339895 | |
dc.subject | Alleles | |
dc.subject | Animals | |
dc.subject | Animals, Genetically Modified | |
dc.subject | Arrestins | |
dc.subject | Drosophila Proteins | |
dc.subject | Drosophila melanogaster | |
dc.subject | Electrophysiology | |
dc.subject | GTP-Binding Protein alpha Subunits, Gq-G11 | |
dc.subject | Light | |
dc.subject | Light Signal Transduction | |
dc.subject | Models, Genetic | |
dc.subject | Mutation | |
dc.subject | Photoreceptor Cells, Invertebrate | |
dc.subject | Receptors, G-Protein-Coupled | |
dc.subject | Retinal Degeneration | |
dc.subject | Rhodopsin | |
dc.subject | Biochemistry, Biophysics, and Structural Biology | |
dc.subject | Neuroscience and Neurobiology | |
dc.title | Protein Gq modulates termination of phototransduction and prevents retinal degeneration | |
dc.type | Journal Article | |
dc.source.journaltitle | The Journal of biological chemistry | |
dc.source.volume | 287 | |
dc.source.issue | 17 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/neurobiology_pp/142 | |
dc.identifier.contextkey | 3608536 | |
html.description.abstract | <p>Appropriate termination of the phototransduction cascade is critical for photoreceptors to achieve high temporal resolution and to prevent excessive Ca(2+)-induced cell toxicity. Using a genetic screen to identify defective photoresponse mutants in Drosophila, we isolated and identified a novel Galpha(q) mutant allele, which has defects in both activation and deactivation. We revealed that G(q) modulates the termination of the light response and that metarhodopsin/G(q) interaction affects subsequent arrestin-rhodopsin (Arr2-Rh1) binding, which mediates the deactivation of metarhodopsin. We further showed that the Galpha(q) mutant undergoes light-dependent retinal degeneration, which is due to the slow accumulation of stable Arr2-Rh1 complexes. Our study revealed the roles of G(q) in mediating photoresponse termination and in preventing retinal degeneration. This pathway may represent a general rapid feedback regulation of G protein-coupled receptor signaling.</p> | |
dc.identifier.submissionpath | neurobiology_pp/142 | |
dc.contributor.department | Graduate School of Biomedical Sciences, Neuroscience Program | |
dc.contributor.department | Li Lab | |
dc.contributor.department | Neurobiology | |
dc.source.pages | 13911-8 | |
dc.contributor.student | Peiyi Guo | |
dc.description.thesisprogram | Neuroscience |