• Login
    View Item 
    •   Home
    • UMass Chan Departments, Programs and Centers
    • Neurobiology
    • Neurobiology Faculty Publications
    • View Item
    •   Home
    • UMass Chan Departments, Programs and Centers
    • Neurobiology
    • Neurobiology Faculty Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of eScholarship@UMassChanCommunitiesPublication DateAuthorsUMass Chan AffiliationsTitlesDocument TypesKeywordsThis CollectionPublication DateAuthorsUMass Chan AffiliationsTitlesDocument TypesKeywords

    My Account

    LoginRegister

    Help

    AboutSubmission GuidelinesData Deposit PolicySearchingAccessibilityTerms of UseWebsite Migration FAQ

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Focal adhesion molecules regulate astrocyte morphology and glutamate transporters to suppress seizure-like behavior

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Cho, Sukhee
    Muthukumar, Allie
    Stork, Tobias
    Coutinho-Budd, Jaeda C.
    Freeman, Marc R.
    UMass Chan Affiliations
    Freeman Lab
    Graduate School of Biomedical Sciences, Neuroscience Program
    Neurobiology
    Document Type
    Journal Article
    Publication Date
    2018-10-30
    Keywords
    Drosophila
    astrocyte
    focal adhesions
    glutamate transporters
    hyperexcitability
    Amino Acids, Peptides, and Proteins
    Cells
    Nervous System
    Nervous System Diseases
    Neuroscience and Neurobiology
    
    Metadata
    Show full item record
    Link to Full Text
    https://doi.org/10.1073/pnas.1800830115
    Abstract
    Astrocytes are important regulators of neural circuit function and behavior in the healthy and diseased nervous system. We screened for molecules in Drosophila astrocytes that modulate neuronal hyperexcitability and identified multiple components of focal adhesion complexes (FAs). Depletion of astrocytic Tensin, beta-integrin, Talin, focal adhesion kinase (FAK), or matrix metalloproteinase 1 (Mmp1), resulted in enhanced behavioral recovery from genetic or pharmacologically induced seizure. Overexpression of Mmp1, predicted to activate FA signaling, led to a reciprocal enhancement of seizure severity. Blockade of FA-signaling molecules in astrocytes at basal levels of CNS excitability resulted in reduced astrocytic coverage of the synaptic neuropil and expression of the excitatory amino acid transporter EAAT1. However, induction of hyperexcitability after depletion of FA-signaling components resulted in enhanced astrocyte coverage and an approximately twofold increase in EAAT1 levels. Our work identifies FA-signaling molecules as important regulators of astrocyte outgrowth and EAAT1 expression under normal physiological conditions. Paradoxically, in the context of hyperexcitability, this pathway negatively regulates astrocytic process outgrowth and EAAT1 expression, and their blockade leading to enhanced recovery from seizure.
    Source

    Proc Natl Acad Sci U S A. 2018 Oct 30;115(44):11316-11321. doi: 10.1073/pnas.1800830115. Epub 2018 Oct 16. Link to article on publisher's site

    DOI
    10.1073/pnas.1800830115
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/37963
    PubMed ID
    30327343
    Related Resources

    Link to Article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1073/pnas.1800830115
    Scopus Count
    Collections
    Neurobiology Student Publications
    Morningside Graduate School of Biomedical Sciences Scholarly Publications
    Neurobiology Faculty Publications

    entitlement

    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Lamar Soutter Library, UMass Chan Medical School | 55 Lake Avenue North | Worcester, MA 01655 USA
    Quick Guide | escholarship@umassmed.edu
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.