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dc.contributor.authorUchigashima, Motokazu
dc.contributor.authorCheung, Amy
dc.contributor.authorSuh, Julie
dc.contributor.authorWatanabe, Masahiko
dc.contributor.authorFutai, Kensuke
dc.date2022-08-11T08:09:29.000
dc.date.accessioned2022-08-23T16:32:49Z
dc.date.available2022-08-23T16:32:49Z
dc.date.issued2019-02-13
dc.date.submitted2019-05-09
dc.identifier.citation<p>J Comp Neurol. 2019 Feb 13. doi: 10.1002/cne.24664. [Epub ahead of print] <a href="https://doi.org/10.1002/cne.24664">Link to article on publisher's site</a></p>
dc.identifier.issn0021-9967 (Linking)
dc.identifier.doi10.1002/cne.24664
dc.identifier.pmid30761534
dc.identifier.urihttp://hdl.handle.net/20.500.14038/37965
dc.description.abstractSynapses, highly specialized membrane junctions between neurons, connect presynaptic neurotransmitter release sites and postsynaptic ligand-gated channels. Neurexins (Nrxns), a family of presynaptic adhesion molecules, have been characterized as major regulators of synapse development and function. Via their extracellular domains, Nrxns bind to different postsynaptic proteins, generating highly diverse functional readouts through their postsynaptic binding partners. Not surprisingly given these versatile protein interactions, mutations and deletions of Nrxn genes have been identified in patients with autism spectrum disorders, intellectual disabilities, and schizophrenia. Therefore, elucidating the expression profiles of Nrxns in the brain is of high significance. Here, using chromogenic and fluorescent in situ hybridization, we characterize the expression patterns of Nrxn isoforms throughout the brain. We found that each Nrxn isoform displays a unique expression profile in a region-, cell type-, and sensory system-specific manner. Interestingly, we also found that alphaNrxn1 and alphaNrxn2 mRNAs are expressed in non-neuronal cells, including astrocytes and oligodendrocytes. Lastly, we found diverse expression patterns of genes that encode Nrxn binding proteins, such as Neuroligins (Nlgns), Leucine-rich repeat transmembrane neuronal protein (Lrrtms) and Latrophilins (Adgrls), suggesting that Nrxn proteins can mediate numerous combinations of trans-synaptic interactions. Together, our anatomical profiling of Nrxn gene expression reflects the diverse roles of Nrxn molecules.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=30761534&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1002/cne.24664
dc.subjectRRID AB_514500
dc.subjectRRID AB_840257
dc.subjectRRIDs
dc.subjectRRID SCR_003070
dc.subjectbrain
dc.subjectin situ hybridization
dc.subjectmice
dc.subjectneurexin
dc.subjectsynapse
dc.subjecttrans-synaptic adhesion
dc.subjectAmino Acids, Peptides, and Proteins
dc.subjectGenetic Phenomena
dc.subjectNervous System
dc.subjectNeurology
dc.subjectNeuroscience and Neurobiology
dc.titleDifferential expression of neurexin genes in the mouse brain
dc.typeJournal Article
dc.source.journaltitleThe Journal of comparative neurology
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/neurobiology_pp/239
dc.identifier.contextkey14467182
html.description.abstract<p>Synapses, highly specialized membrane junctions between neurons, connect presynaptic neurotransmitter release sites and postsynaptic ligand-gated channels. Neurexins (Nrxns), a family of presynaptic adhesion molecules, have been characterized as major regulators of synapse development and function. Via their extracellular domains, Nrxns bind to different postsynaptic proteins, generating highly diverse functional readouts through their postsynaptic binding partners. Not surprisingly given these versatile protein interactions, mutations and deletions of Nrxn genes have been identified in patients with autism spectrum disorders, intellectual disabilities, and schizophrenia. Therefore, elucidating the expression profiles of Nrxns in the brain is of high significance. Here, using chromogenic and fluorescent in situ hybridization, we characterize the expression patterns of Nrxn isoforms throughout the brain. We found that each Nrxn isoform displays a unique expression profile in a region-, cell type-, and sensory system-specific manner. Interestingly, we also found that alphaNrxn1 and alphaNrxn2 mRNAs are expressed in non-neuronal cells, including astrocytes and oligodendrocytes. Lastly, we found diverse expression patterns of genes that encode Nrxn binding proteins, such as Neuroligins (Nlgns), Leucine-rich repeat transmembrane neuronal protein (Lrrtms) and Latrophilins (Adgrls), suggesting that Nrxn proteins can mediate numerous combinations of trans-synaptic interactions. Together, our anatomical profiling of Nrxn gene expression reflects the diverse roles of Nrxn molecules.</p>
dc.identifier.submissionpathneurobiology_pp/239
dc.contributor.departmentGraduate School of Biomedical Sciences, MD/PhD Program
dc.contributor.departmentFutai Lab
dc.contributor.departmentNeurobiology
dc.contributor.departmentBrudnick Neuropsychiatric Research Institute
dc.contributor.studentAmy Cheung
dc.description.thesisprogramMD/PhD


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