• Login
    View Item 
    •   Home
    • UMass Chan Departments, Programs and Centers
    • Neurobiology
    • Neurobiology Faculty Publications
    • View Item
    •   Home
    • UMass Chan Departments, Programs and Centers
    • Neurobiology
    • Neurobiology Faculty Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of eScholarship@UMassChanCommunitiesPublication DateAuthorsUMass Chan AffiliationsTitlesDocument TypesKeywordsThis CollectionPublication DateAuthorsUMass Chan AffiliationsTitlesDocument TypesKeywords

    My Account

    LoginRegister

    Help

    AboutSubmission GuidelinesData Deposit PolicySearchingAccessibilityTerms of UseWebsite Migration FAQ

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Neuronal vulnerability and multilineage diversity in multiple sclerosis

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Schirmer, Lucas
    Werneburg, Sebastian
    Schafer, Dorothy P
    Kriegstein, Arnold R.
    Rowitch, David H.
    UMass Chan Affiliations
    Schafer Lab
    Brudnick Neuropsychiatric Research Institute
    Neurobiology
    Document Type
    Journal Article
    Publication Date
    2019-07-17
    Keywords
    Multiple sclerosis
    Neuroimmunology
    Immune System Diseases
    Immunopathology
    Nervous System
    Nervous System Diseases
    Neuroscience and Neurobiology
    Nucleic Acids, Nucleotides, and Nucleosides
    
    Metadata
    Show full item record
    Link to Full Text
    https://doi.org/10.1038/s41586-019-1404-z
    Abstract
    Multiple sclerosis (MS) is a neuroinflammatory disease with a relapsing-remitting disease course at early stages, distinct lesion characteristics in cortical grey versus subcortical white matter and neurodegeneration at chronic stages. Here we used single-nucleus RNA sequencing to assess changes in expression in multiple cell lineages in MS lesions and validated the results using multiplex in situ hybridization. We found selective vulnerability and loss of excitatory CUX2-expressing projection neurons in upper-cortical layers underlying meningeal inflammation; such MS neuron populations exhibited upregulation of stress pathway genes and long non-coding RNAs. Signatures of stressed oligodendrocytes, reactive astrocytes and activated microglia mapped most strongly to the rim of MS plaques. Notably, single-nucleus RNA sequencing identified phagocytosing microglia and/or macrophages by their ingestion and perinuclear import of myelin transcripts, confirmed by functional mouse and human culture assays. Our findings indicate lineage- and region-specific transcriptomic changes associated with selective cortical neuron damage and glial activation contributing to progression of MS lesions.
    Source

    Nature. 2019 Jul 17. doi: 10.1038/s41586-019-1404-z. [Epub ahead of print] Link to article on publisher's site

    DOI
    10.1038/s41586-019-1404-z
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/37973
    PubMed ID
    31316211
    Notes

    Full author list omitted for brevity. For the full list of authors, see article.

    Related Resources

    Link to Article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1038/s41586-019-1404-z
    Scopus Count
    Collections
    Neurobiology Faculty Publications

    entitlement

    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Lamar Soutter Library, UMass Chan Medical School | 55 Lake Avenue North | Worcester, MA 01655 USA
    Quick Guide | escholarship@umassmed.edu
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.