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dc.contributor.authorKim, Soo Mi
dc.contributor.authorPower, Andrea
dc.contributor.authorCoon, Steven L.
dc.contributor.authorConstance, Cara M.
dc.contributor.authorNishimura, Takuya
dc.contributor.authorHirai, Hiroki
dc.contributor.authorCai, Tao
dc.contributor.authorEisner, Christoph
dc.contributor.authorWeaver, David R.
dc.contributor.authorPiggins, Hugh D.
dc.contributor.authorKlein, David C.
dc.contributor.authorSchnermann, Jurgen
dc.contributor.authorNotkins, Abner L.
dc.date2022-08-11T08:09:29.000
dc.date.accessioned2022-08-23T16:32:57Z
dc.date.available2022-08-23T16:32:57Z
dc.date.issued2009-09-01
dc.date.submitted2012-05-24
dc.identifier.citationFASEB J. 2009 Sep;23(9):3226-32. Epub 2009 May 11. <a href="http://dx.doi.org/10.1096/fj.09-132019">Link to article on publisher's site</a>
dc.identifier.issn0892-6638 (Linking)
dc.identifier.doi10.1096/fj.09-132019
dc.identifier.pmid19433624
dc.identifier.urihttp://hdl.handle.net/20.500.14038/37992
dc.description.abstractTargeted deletion of IA-2 and IA-2beta, major autoantigens in type 1 diabetes and transmembrane secretory vesicle proteins, results in impaired secretion of hormones and neurotransmitters. In the present study, we evaluated the effect of these deletions on daily rhythms in blood pressure, heart rate, core body temperature, and spontaneous physical and neuronal activity. We found that deletion of both IA-2 and IA-2beta profoundly disrupts the usual diurnal variation of each of these parameters, whereas the deletion of either IA-2 or IA-2beta alone did not produce a major change. In situ hybridization revealed that IA-2 and IA-2beta transcripts are highly but nonrhythmically expressed in the suprachiasmatic nuclei, the site of the brain's master circadian oscillator. Electrophysiological studies on tissue slices from the suprachiasmatic nuclei showed that disruption of both IA-2 and IA-2beta results in significant alterations in neuronal firing. From these studies, we concluded that deletion of IA-2 and IA-2beta, structural proteins of secretory vesicles and modulators of neuroendocrine secretion, has a profound effect on the circadian system.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=19433624&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1096/fj.09-132019
dc.subjectAnimals
dc.subject*Circadian Rhythm
dc.subject*Electrophysiology
dc.subjectHemodynamics
dc.subjectMice
dc.subjectRNA, Messenger
dc.subjectReceptor-Like Protein Tyrosine Phosphatases, Class
dc.subject8
dc.subjectSecretory Vesicles
dc.subjectSuprachiasmatic Nucleus
dc.subjectNeuroscience and Neurobiology
dc.titleDeletion of the secretory vesicle proteins IA-2 and IA-2beta disrupts circadian rhythms of cardiovascular and physical activity
dc.typeJournal Article
dc.source.journaltitleThe FASEB journal : official publication of the Federation of American Societies for Experimental Biology
dc.source.volume23
dc.source.issue9
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/neurobiology_pp/27
dc.identifier.contextkey2911142
html.description.abstract<p>Targeted deletion of IA-2 and IA-2beta, major autoantigens in type 1 diabetes and transmembrane secretory vesicle proteins, results in impaired secretion of hormones and neurotransmitters. In the present study, we evaluated the effect of these deletions on daily rhythms in blood pressure, heart rate, core body temperature, and spontaneous physical and neuronal activity. We found that deletion of both IA-2 and IA-2beta profoundly disrupts the usual diurnal variation of each of these parameters, whereas the deletion of either IA-2 or IA-2beta alone did not produce a major change. In situ hybridization revealed that IA-2 and IA-2beta transcripts are highly but nonrhythmically expressed in the suprachiasmatic nuclei, the site of the brain's master circadian oscillator. Electrophysiological studies on tissue slices from the suprachiasmatic nuclei showed that disruption of both IA-2 and IA-2beta results in significant alterations in neuronal firing. From these studies, we concluded that deletion of IA-2 and IA-2beta, structural proteins of secretory vesicles and modulators of neuroendocrine secretion, has a profound effect on the circadian system.</p>
dc.identifier.submissionpathneurobiology_pp/27
dc.contributor.departmentWeaver Lab
dc.contributor.departmentNeurobiology
dc.source.pages3226-32


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