RNase protection assay

Authors
Emery, Patrick
UMass Chan Affiliations
Emery Lab
Neurobiology
Document Type
Book Chapter
Publication Date
2007-02-02
Keywords
Alternative Splicing
Antisense Elements (Genetics)
Bacteriophage T7
Circadian Rhythm
DNA-Directed RNA Polymerases
Molecular Probe Techniques
Promoter Regions, Genetic
RNA, Messenger
RNA, Viral
*Ribonucleases

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Link to Full Text
http://dx.doi.org/10.1007/978-1-59745-257-1_24
Abstract
The RNase protection assay is a standard approach to determine mRNA levels of a gene of interest in different tissues, developmental stages, or times of the day. Splicing or promoter variants can be studied with specific probes. It is widely used in chronobiology to study the temporal profile of expression of circadian genes and the effects of genetic manipulation on these oscillations. Methods to generate the riboprobes and to perform the RNase protection assay itself are described in this chapter.
Source
Methods Mol Biol. 2007;362:343-8. DOI 10.1007/978-1-59745-257-1_24
DOI
10.1007/978-1-59745-257-1_24
Permanent Link to this Item
http://hdl.handle.net/20.500.14038/38019
PubMed ID
17417021
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