Sleep rhythmicity and homeostasis in mice with targeted disruption of mPeriod genes
Authors
Shiromani, Priyattam J.Xu, Man
Winston, Elizabeth M.
Shiromani, Samara N.
Geraschenko, Dmitry
Weaver, David R.
Document Type
Journal ArticlePublication Date
2004-07-01Keywords
AllelesAnimals
Body Temperature
Cell Cycle Proteins
Circadian Rhythm
Electrodes, Implanted
Electroencephalography
Electromyography
Genotype
Homeostasis
Male
Mice
Motor Activity
Mutation
Nuclear Proteins
Period Circadian Proteins
*Periodicity
Reverse Transcriptase Polymerase Chain Reaction
Sleep
Sleep, REM
Transcription Factors
Wakefulness
Neuroscience and Neurobiology
Metadata
Show full item recordAbstract
In mammals, sleep is regulated by circadian and homeostatic mechanisms. The circadian component, residing in the suprachiasmatic nucleus (SCN), regulates the timing of sleep, whereas homeostatic factors determine the amount of sleep. It is believed that these two processes regulating sleep are independent because sleep amount is unchanged after SCN lesions. However, because such lesions necessarily damage neuronal connectivity, it is preferable to investigate this question in a genetic model that overcomes the confounding influence of circadian rhythmicity. Mice with disruption of both mouse Period genes (mPer)1 and mPer2 have a robust diurnal sleep-wake rhythm in an entrained light-dark cycle but lose rhythmicity in a free-run condition. Here, we examine the role of the mPer genes on the rhythmic and homeostatic regulation of sleep. In entrained conditions, when averaged over the 24-h period, there were no significant differences in waking, slow-wave sleep (SWS), or rapid eye movement (REM) sleep between mPer1, mPer2, mPer3, mPer1-mPer2 double-mutant, and wild-type mice. The mice were then kept awake for 6 h (light period 6-12), and the mPer mutants exhibited increased sleep drive, indicating an intact sleep homeostatic response in the absence of the mPer genes. In free-run conditions (constant darkness), the mPer1-mPer2 double mutants became arrhythmic, but they continued to maintain their sleep levels even after 36 days in free-running conditions. Although mPer1 and mPer2 represent key elements of the molecular clock in the SCN, they are not required for homeostatic regulation of the daily amounts of waking, SWS, or REM sleep.Source
Am J Physiol Regul Integr Comp Physiol. 2004 Jul;287(1):R47-57. Epub 2004 Mar 18. Link to article on publisher's websiteDOI
10.1152/ajpregu.00138.2004Permanent Link to this Item
http://hdl.handle.net/20.500.14038/38044PubMed ID
15031135Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1152/ajpregu.00138.2004