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dc.contributor.authorShiromani, Priyattam J.
dc.contributor.authorXu, Man
dc.contributor.authorWinston, Elizabeth M.
dc.contributor.authorShiromani, Samara N.
dc.contributor.authorGeraschenko, Dmitry
dc.contributor.authorWeaver, David R.
dc.date2022-08-11T08:09:30.000
dc.date.accessioned2022-08-23T16:33:10Z
dc.date.available2022-08-23T16:33:10Z
dc.date.issued2004-07-01
dc.date.submitted2012-05-24
dc.identifier.citationAm J Physiol Regul Integr Comp Physiol. 2004 Jul;287(1):R47-57. Epub 2004 Mar 18. <a href="http://dx.doi.org/10.1152/ajpregu.00138.2004">Link to article on publisher's website</a>
dc.identifier.issn0363-6119 (Linking)
dc.identifier.doi10.1152/ajpregu.00138.2004
dc.identifier.pmid15031135
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38044
dc.description.abstractIn mammals, sleep is regulated by circadian and homeostatic mechanisms. The circadian component, residing in the suprachiasmatic nucleus (SCN), regulates the timing of sleep, whereas homeostatic factors determine the amount of sleep. It is believed that these two processes regulating sleep are independent because sleep amount is unchanged after SCN lesions. However, because such lesions necessarily damage neuronal connectivity, it is preferable to investigate this question in a genetic model that overcomes the confounding influence of circadian rhythmicity. Mice with disruption of both mouse Period genes (mPer)1 and mPer2 have a robust diurnal sleep-wake rhythm in an entrained light-dark cycle but lose rhythmicity in a free-run condition. Here, we examine the role of the mPer genes on the rhythmic and homeostatic regulation of sleep. In entrained conditions, when averaged over the 24-h period, there were no significant differences in waking, slow-wave sleep (SWS), or rapid eye movement (REM) sleep between mPer1, mPer2, mPer3, mPer1-mPer2 double-mutant, and wild-type mice. The mice were then kept awake for 6 h (light period 6-12), and the mPer mutants exhibited increased sleep drive, indicating an intact sleep homeostatic response in the absence of the mPer genes. In free-run conditions (constant darkness), the mPer1-mPer2 double mutants became arrhythmic, but they continued to maintain their sleep levels even after 36 days in free-running conditions. Although mPer1 and mPer2 represent key elements of the molecular clock in the SCN, they are not required for homeostatic regulation of the daily amounts of waking, SWS, or REM sleep.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=15031135&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1152/ajpregu.00138.2004
dc.subjectAlleles
dc.subjectAnimals
dc.subjectBody Temperature
dc.subjectCell Cycle Proteins
dc.subjectCircadian Rhythm
dc.subjectElectrodes, Implanted
dc.subjectElectroencephalography
dc.subjectElectromyography
dc.subjectGenotype
dc.subjectHomeostasis
dc.subjectMale
dc.subjectMice
dc.subjectMotor Activity
dc.subjectMutation
dc.subjectNuclear Proteins
dc.subjectPeriod Circadian Proteins
dc.subject*Periodicity
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectSleep
dc.subjectSleep, REM
dc.subjectTranscription Factors
dc.subjectWakefulness
dc.subjectNeuroscience and Neurobiology
dc.titleSleep rhythmicity and homeostasis in mice with targeted disruption of mPeriod genes
dc.typeJournal Article
dc.source.journaltitleAmerican journal of physiology. Regulatory, integrative and comparative physiology 15191924
dc.source.volume287
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/neurobiology_pp/77
dc.identifier.contextkey2911192
html.description.abstract<p>In mammals, sleep is regulated by circadian and homeostatic mechanisms. The circadian component, residing in the suprachiasmatic nucleus (SCN), regulates the timing of sleep, whereas homeostatic factors determine the amount of sleep. It is believed that these two processes regulating sleep are independent because sleep amount is unchanged after SCN lesions. However, because such lesions necessarily damage neuronal connectivity, it is preferable to investigate this question in a genetic model that overcomes the confounding influence of circadian rhythmicity. Mice with disruption of both mouse Period genes (mPer)1 and mPer2 have a robust diurnal sleep-wake rhythm in an entrained light-dark cycle but lose rhythmicity in a free-run condition. Here, we examine the role of the mPer genes on the rhythmic and homeostatic regulation of sleep. In entrained conditions, when averaged over the 24-h period, there were no significant differences in waking, slow-wave sleep (SWS), or rapid eye movement (REM) sleep between mPer1, mPer2, mPer3, mPer1-mPer2 double-mutant, and wild-type mice. The mice were then kept awake for 6 h (light period 6-12), and the mPer mutants exhibited increased sleep drive, indicating an intact sleep homeostatic response in the absence of the mPer genes. In free-run conditions (constant darkness), the mPer1-mPer2 double mutants became arrhythmic, but they continued to maintain their sleep levels even after 36 days in free-running conditions. Although mPer1 and mPer2 represent key elements of the molecular clock in the SCN, they are not required for homeostatic regulation of the daily amounts of waking, SWS, or REM sleep.</p>
dc.identifier.submissionpathneurobiology_pp/77
dc.contributor.departmentWeaver Lab
dc.contributor.departmentNeurobiology
dc.source.pagesR47-57


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