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dc.contributor.authorNickerson, Jeffrey A.
dc.date2022-08-11T08:09:30.000
dc.date.accessioned2022-08-23T16:33:24Z
dc.date.available2022-08-23T16:33:24Z
dc.date.issued2009-01-23
dc.date.submitted2011-02-23
dc.identifier.citationRNA Biol. 2009 Jan-Mar;6(1):25-30. Epub 2009 Jan 2. <a href="http://dx.doi.org/10.4161/rna.6.1.7563">Link to article on publisher's website</a>
dc.identifier.issn1547-6286 (Linking)
dc.identifier.doi10.4161/rna.6.1.7563
dc.identifier.pmid19098457
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38099
dc.description.abstractIn vitro assays have contributed important insights into the mechanisms of RNA metabolism in cells. A growing collection of microscopy techniques is allowing the measurement of macromolecular binding and complex formation in the context of a real cell. We will first discuss two of the more established techniques. Fluorescence resonance energy transfer (FRET) identifies binding partners, pairs of molecules residing in the same macromolecular complexes. The complimentary technique of fluorescence recovery after photobleaching (FRAP) measures the rates of binding and unbinding of those molecules in their complexes. A newer technique--in vitro FRAP--assesses the regulation of binding and complex formation by co-factors in the nucleus.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=19098457&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectAdenosine Triphosphate
dc.subjectAnimals
dc.subjectBiochemistry
dc.subjectFluorescence Recovery After Photobleaching
dc.subjectFluorescence Resonance Energy Transfer
dc.subjectHumans
dc.subjectKinetics
dc.subjectMacromolecular Substances
dc.subjectMicroscopy
dc.subjectModels, Biological
dc.subjectMolecular Biology
dc.subjectProtein Binding
dc.subjectRNA
dc.subjectCell Biology
dc.titleThe biochemistry of RNA metabolism studied in situ
dc.typeJournal Article
dc.source.journaltitleRNA biology
dc.source.volume6
dc.source.issue1
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1001&amp;context=nickerson&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/nickerson/2
dc.identifier.contextkey1809756
refterms.dateFOA2022-08-23T16:33:25Z
html.description.abstract<p>In vitro assays have contributed important insights into the mechanisms of RNA metabolism in cells. A growing collection of microscopy techniques is allowing the measurement of macromolecular binding and complex formation in the context of a real cell. We will first discuss two of the more established techniques. Fluorescence resonance energy transfer (FRET) identifies binding partners, pairs of molecules residing in the same macromolecular complexes. The complimentary technique of fluorescence recovery after photobleaching (FRAP) measures the rates of binding and unbinding of those molecules in their complexes. A newer technique--in vitro FRAP--assesses the regulation of binding and complex formation by co-factors in the nucleus.</p>
dc.identifier.submissionpathnickerson/2
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages25-30


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