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dc.contributor.authorTafuri, Anna
dc.contributor.authorBowers, William E.
dc.contributor.authorHandler, Eugene S.
dc.contributor.authorAppel, Michael C.
dc.contributor.authorLew, Robert A.
dc.contributor.authorGreiner, Dale L.
dc.contributor.authorMordes, John P.
dc.contributor.authorRossini, Aldo A.
dc.date2022-08-11T08:09:30.000
dc.date.accessioned2022-08-23T16:33:29Z
dc.date.available2022-08-23T16:33:29Z
dc.date.issued1993-05-01
dc.date.submitted2008-10-31
dc.identifier.citationJ Clin Invest. 1993 May;91(5):2040-8. <a href="http://dx.doi.org/10.1172/JCI116426">Link to article on publisher's site</a>
dc.identifier.issn0021-9738 (Print)
dc.identifier.doi10.1172/JCI116426
dc.identifier.pmid8486773
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38118
dc.description.abstractDendritic cells (DC) present antigen and initiate T cell-mediated immune responses. To investigate the possible association of autoimmunity with DC function, we compared the accessory activity of splenic DC from Wistar/Furth (WF) and diabetes-prone (DP) BioBreeding (BB) rats. The latter develop autoimmune diabetes and thyroiditis. DC function was quantified in vitro by measuring T cell proliferation in mitogen-stimulated and mixed lymphocyte reactions. When purified without macrophage coculture, WF and DP DC displayed similar levels of accessory activity. In contrast, when purified by a method involving coculture with macrophages, DC from DP rats consistently displayed greater accessory activity. This finding could not be explained by morphological or phenotypic differences between DP and WF DC. In accessory activity assays performed after reciprocal DC cocultures with DP and WF macrophages, DP DC exhibited higher accessory activity irrespective of macrophage donor strain. We also compared the accessory activity of WF and DP DC cultured in the presence of conditioned medium and a mixture of IL-1 and GM-CSF. In all assays, DP DC exhibited higher accessory activity. In studies of (WF x DP) F1 hybrids, the high accessory activity of DP DC was observed to be heritable, and studies of WF and DP radiation chimeras indicated that the effect was an intrinsic property of the DP hematopoietic system. We conclude: (a) splenic DC from DP and WF rats possess similar basal levels of accessory potency; (b) after interaction with macrophages, DC of DP origin are capable of greater stimulatory activity than are WF DC; and (c) the mechanism responsible for this phenomenon involves differential responsiveness of DP and WF DC to macrophage-derived factors such as IL-1 and GM-CSF.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=8486773&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectAnimals
dc.subjectCell Communication
dc.subjectCells, Cultured
dc.subjectCulture Media, Conditioned
dc.subjectCytokines
dc.subjectDendritic Cells
dc.subjectFemale
dc.subjectGranulocyte-Macrophage Colony-Stimulating Factor
dc.subjectHumans
dc.subjectInterleukin-1
dc.subject*Lymphocyte Activation
dc.subjectLymphocyte Culture Test, Mixed
dc.subjectMacrophages
dc.subjectMale
dc.subjectMice
dc.subjectRats
dc.subjectRats, Inbred BB
dc.subjectRats, Inbred WF
dc.subjectRecombinant Proteins
dc.subjectSpecies Specificity
dc.subjectSpleen
dc.subjectT-Lymphocytes
dc.subjectThymidine
dc.subjectEndocrinology, Diabetes, and Metabolism
dc.subjectMedical Pathology
dc.subjectPathology
dc.titleHigh stimulatory activity of dendritic cells from diabetes-prone BioBreeding/Worcester rats exposed to macrophage-derived factors
dc.typeArticle
dc.source.journaltitleThe Journal of clinical investigation
dc.source.volume91
dc.source.issue5
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2003&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/1004
dc.identifier.contextkey659187
refterms.dateFOA2022-08-23T16:33:29Z
html.description.abstract<p>Dendritic cells (DC) present antigen and initiate T cell-mediated immune responses. To investigate the possible association of autoimmunity with DC function, we compared the accessory activity of splenic DC from Wistar/Furth (WF) and diabetes-prone (DP) BioBreeding (BB) rats. The latter develop autoimmune diabetes and thyroiditis. DC function was quantified in vitro by measuring T cell proliferation in mitogen-stimulated and mixed lymphocyte reactions. When purified without macrophage coculture, WF and DP DC displayed similar levels of accessory activity. In contrast, when purified by a method involving coculture with macrophages, DC from DP rats consistently displayed greater accessory activity. This finding could not be explained by morphological or phenotypic differences between DP and WF DC. In accessory activity assays performed after reciprocal DC cocultures with DP and WF macrophages, DP DC exhibited higher accessory activity irrespective of macrophage donor strain. We also compared the accessory activity of WF and DP DC cultured in the presence of conditioned medium and a mixture of IL-1 and GM-CSF. In all assays, DP DC exhibited higher accessory activity. In studies of (WF x DP) F1 hybrids, the high accessory activity of DP DC was observed to be heritable, and studies of WF and DP radiation chimeras indicated that the effect was an intrinsic property of the DP hematopoietic system. We conclude: (a) splenic DC from DP and WF rats possess similar basal levels of accessory potency; (b) after interaction with macrophages, DC of DP origin are capable of greater stimulatory activity than are WF DC; and (c) the mechanism responsible for this phenomenon involves differential responsiveness of DP and WF DC to macrophage-derived factors such as IL-1 and GM-CSF.</p>
dc.identifier.submissionpathoapubs/1004
dc.contributor.departmentDepartment of Pathology
dc.contributor.departmentDepartment of Medicine, Division of Diabetes
dc.source.pages2040-8


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