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Monoclonal antibodies for detection of Norwalk virus antigen in stools

dc.contributor.authorHerrmann, John E.
dc.contributor.authorBlacklow, Neil R.
dc.contributor.authorMatsui, Suzanne M.
dc.contributor.authorLewis, Terry L.
dc.contributor.authorEstes, Mary K.
dc.contributor.authorBall, Judith M.
dc.contributor.authorBrinker, James P.
dc.date2022-08-11T08:09:30.000
dc.date.accessioned2022-08-23T16:33:33Z
dc.date.available2022-08-23T16:33:33Z
dc.date.issued1995-09-01
dc.date.submitted2008-10-31
dc.identifier.citationJ Clin Microbiol. 1995 Sep;33(9):2511-3.
dc.identifier.issn0095-1137 (Print)
dc.identifier.pmid7494063
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38134
dc.description.abstractMonoclonal antibodies against the prototype 8FIIa strain of Norwalk virus were prepared and applied to an enzyme immunoassay (EIA) for detecting Norwalk virus in stool specimens. The monoclonal antibodies immunoprecipitated a 58-kDa protein which had been produced by in vitro transcription-translation of Norwalk virus cloned cDNA, and they reacted by EIA with recombinant Norwalk virus capsid protein at a sensitivity level of 1 ng/ml. The EIA detected virus in all tested samples from 15 different Norwalk virus-infected volunteers. No cross-reactions were seen in stools containing other caliciviruses or in stools containing rotaviruses, astroviruses, or enteric adenoviruses.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=7494063&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectAntibodies, Monoclonal
dc.subjectAntigens, Viral
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectFeces
dc.subjectHumans
dc.subjectNorwalk virus
dc.subjectInfectious Disease
dc.subjectMedical Immunology
dc.titleMonoclonal antibodies for detection of Norwalk virus antigen in stools
dc.typeJournal Article
dc.source.journaltitleJournal of clinical microbiology
dc.source.volume33
dc.source.issue9
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2018&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/1019
dc.identifier.contextkey659203
refterms.dateFOA2022-08-23T16:33:34Z
html.description.abstract<p>Monoclonal antibodies against the prototype 8FIIa strain of Norwalk virus were prepared and applied to an enzyme immunoassay (EIA) for detecting Norwalk virus in stool specimens. The monoclonal antibodies immunoprecipitated a 58-kDa protein which had been produced by in vitro transcription-translation of Norwalk virus cloned cDNA, and they reacted by EIA with recombinant Norwalk virus capsid protein at a sensitivity level of 1 ng/ml. The EIA detected virus in all tested samples from 15 different Norwalk virus-infected volunteers. No cross-reactions were seen in stools containing other caliciviruses or in stools containing rotaviruses, astroviruses, or enteric adenoviruses.</p>
dc.identifier.submissionpathoapubs/1019
dc.contributor.departmentDivision of Infectious Diseases and Immunology
dc.source.pages2511-3


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