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dc.contributor.authorNishioka, Walter K.
dc.contributor.authorWelsh, Raymond M.
dc.date2022-08-11T08:09:30.000
dc.date.accessioned2022-08-23T16:33:42Z
dc.date.available2022-08-23T16:33:42Z
dc.date.issued1992-01-01
dc.date.submitted2008-10-31
dc.identifier.citationJ Exp Med. 1992 Jan 1;175(1):23-7.
dc.identifier.issn0022-1007 (Print)
dc.identifier.pmid1309853
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38166
dc.description.abstractCytotoxic T lymphocytes (CTL) kill their target cells via a contact-dependent mechanism that results in the perturbation of the target cell's plasma membrane and the fragmentation of the target cell's DNA into nucleosomal particles. The membrane disruption is presumed to be due to the action of perforin, while the DNA fragmentation is thought to be by the activation of an endogenous nuclease(s). DNA topoisomerases I and II are nuclear enzymes with inherent endonuclease activities. We have investigated their role in the CTL-induced DNA fragmentation process. We report that in CTL killing assays, the treatment of target cells with topoisomerase I and II inhibitors blocks the CTL-induced DNA fragmentation process, but not the lysis of the target cell.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=1309853&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectAmsacrine
dc.subjectAnimals
dc.subjectCamptothecin
dc.subjectCells, Cultured
dc.subjectCytotoxicity, Immunologic
dc.subjectDNA
dc.subject*DNA Damage
dc.subjectDNA Topoisomerases, Type I
dc.subjectDNA Topoisomerases, Type II
dc.subjectFemale
dc.subjectKiller Cells, Natural
dc.subjectLymphocyte Depletion
dc.subjectLymphocytic choriomeningitis virus
dc.subjectMale
dc.subjectMice
dc.subjectMice, Inbred Strains
dc.subjectT-Lymphocytes, Cytotoxic
dc.subjectTeniposide
dc.subjectImmunology and Infectious Disease
dc.subjectMedical Pathology
dc.titleInhibition of cytotoxic T lymphocyte-induced target cell DNA fragmentation, but not lysis, by inhibitors of DNA topoisomerases I and II
dc.typeJournal Article
dc.source.journaltitleThe Journal of experimental medicine
dc.source.volume175
dc.source.issue1
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2047&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/1048
dc.identifier.contextkey659236
refterms.dateFOA2022-08-23T16:33:42Z
html.description.abstract<p>Cytotoxic T lymphocytes (CTL) kill their target cells via a contact-dependent mechanism that results in the perturbation of the target cell's plasma membrane and the fragmentation of the target cell's DNA into nucleosomal particles. The membrane disruption is presumed to be due to the action of perforin, while the DNA fragmentation is thought to be by the activation of an endogenous nuclease(s). DNA topoisomerases I and II are nuclear enzymes with inherent endonuclease activities. We have investigated their role in the CTL-induced DNA fragmentation process. We report that in CTL killing assays, the treatment of target cells with topoisomerase I and II inhibitors blocks the CTL-induced DNA fragmentation process, but not the lysis of the target cell.</p>
dc.identifier.submissionpathoapubs/1048
dc.contributor.departmentDepartment of Pathology
dc.source.pages23-7


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