Identification and analysis for cross-reactivity among hantaviruses of H-2b-restricted cytotoxic T-lymphocyte epitopes in Sin Nombre virus nucleocapsid protein
Authors
Maeda, KenWest, Kim
Toyosaki-Maeda, Tomoko
Rothman, Alan L.
Ennis, Francis A.
Terajima, Masanori
UMass Chan Affiliations
Center for Infectious Disease and Vaccine ResearchDocument Type
Journal ArticlePublication Date
2004-06-26Keywords
Amino Acid SequenceAnimals
Antibody Specificity
Cell Line, Tumor
Cross Reactions
Epitopes
H-2 Antigens
Hantavirus
Hantavirus Pulmonary Syndrome
Humans
Isoantigens
Major Histocompatibility Complex
Mice
Molecular Sequence Data
Nucleocapsid
Nucleocapsid Proteins
Peptide Fragments
Sin Nombre virus
T-Lymphocytes, Cytotoxic
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Sin Nombre virus (SNV) causes hantavirus pulmonary syndrome (HPS), with a high rate of mortality in humans who are infected by the transmission of virus from the natural rodent host. In humans, cytotoxic T lymphocytes (CTL) specific for SNV appear to play an important role in the pathogenicity of HPS. There is a correlation between the frequencies of SNV-specific CTLs and the severity of HPS disease. In order to create a mouse model to study the role of SNV-specific T cells in vivo, T cell responses to SNV nucleocapsid (N) protein in B6.PL Thy1(a)/Cy mice (H-2(b)) immunized with plasmid DNA or recombinant vaccinia virus expressing SNV N protein were examined. Four peptides, NC94-101, NC175-189, NC217-231 and NC331-345, were recognized by CD8(+) T cells in CTL and ELISPOT assays in SNV N-immunized mice. Interestingly, two of these epitopes are located in the central region of the SNV N protein, where several human CD8(+) T-cell epitopes have been defined in Puumala virus and SNV. CTL lines specific for these four epitopes were cross-reactive to corresponding Puumala virus peptides, but only one of them was cross-reactive to Hantaan virus peptides. These results will enable the analysis of the roles of CTL in immunopathology of HPS in experimental mouse models of HPS.Source
J Gen Virol. 2004 Jul;85(Pt 7):1909-19. Link to article on publisher's siteDOI
10.1099/vir.0.79945-0Permanent Link to this Item
http://hdl.handle.net/20.500.14038/38191PubMed ID
15218176Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1099/vir.0.79945-0