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dc.contributor.authorMaeda, Ken
dc.contributor.authorWest, Kim
dc.contributor.authorToyosaki-Maeda, Tomoko
dc.contributor.authorRothman, Alan L.
dc.contributor.authorEnnis, Francis A.
dc.contributor.authorTerajima, Masanori
dc.date2022-08-11T08:09:31.000
dc.date.accessioned2022-08-23T16:33:49Z
dc.date.available2022-08-23T16:33:49Z
dc.date.issued2004-06-26
dc.date.submitted2008-10-31
dc.identifier.citationJ Gen Virol. 2004 Jul;85(Pt 7):1909-19. <a href="http://dx.doi.org/10.1099/vir.0.79945-0">Link to article on publisher's site</a>
dc.identifier.issn0022-1317 (Print)
dc.identifier.doi10.1099/vir.0.79945-0
dc.identifier.pmid15218176
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38191
dc.description.abstractSin Nombre virus (SNV) causes hantavirus pulmonary syndrome (HPS), with a high rate of mortality in humans who are infected by the transmission of virus from the natural rodent host. In humans, cytotoxic T lymphocytes (CTL) specific for SNV appear to play an important role in the pathogenicity of HPS. There is a correlation between the frequencies of SNV-specific CTLs and the severity of HPS disease. In order to create a mouse model to study the role of SNV-specific T cells in vivo, T cell responses to SNV nucleocapsid (N) protein in B6.PL Thy1(a)/Cy mice (H-2(b)) immunized with plasmid DNA or recombinant vaccinia virus expressing SNV N protein were examined. Four peptides, NC94-101, NC175-189, NC217-231 and NC331-345, were recognized by CD8(+) T cells in CTL and ELISPOT assays in SNV N-immunized mice. Interestingly, two of these epitopes are located in the central region of the SNV N protein, where several human CD8(+) T-cell epitopes have been defined in Puumala virus and SNV. CTL lines specific for these four epitopes were cross-reactive to corresponding Puumala virus peptides, but only one of them was cross-reactive to Hantaan virus peptides. These results will enable the analysis of the roles of CTL in immunopathology of HPS in experimental mouse models of HPS.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=15218176&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1099/vir.0.79945-0
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectAntibody Specificity
dc.subjectCell Line, Tumor
dc.subjectCross Reactions
dc.subjectEpitopes
dc.subjectH-2 Antigens
dc.subjectHantavirus
dc.subjectHantavirus Pulmonary Syndrome
dc.subjectHumans
dc.subjectIsoantigens
dc.subjectMajor Histocompatibility Complex
dc.subjectMice
dc.subjectMolecular Sequence Data
dc.subjectNucleocapsid
dc.subjectNucleocapsid Proteins
dc.subjectPeptide Fragments
dc.subjectSin Nombre virus
dc.subjectT-Lymphocytes, Cytotoxic
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleIdentification and analysis for cross-reactivity among hantaviruses of H-2b-restricted cytotoxic T-lymphocyte epitopes in Sin Nombre virus nucleocapsid protein
dc.typeJournal Article
dc.source.journaltitleThe Journal of general virology
dc.source.volume85
dc.source.issuePt 7
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/1071
dc.identifier.contextkey659260
html.description.abstract<p>Sin Nombre virus (SNV) causes hantavirus pulmonary syndrome (HPS), with a high rate of mortality in humans who are infected by the transmission of virus from the natural rodent host. In humans, cytotoxic T lymphocytes (CTL) specific for SNV appear to play an important role in the pathogenicity of HPS. There is a correlation between the frequencies of SNV-specific CTLs and the severity of HPS disease. In order to create a mouse model to study the role of SNV-specific T cells in vivo, T cell responses to SNV nucleocapsid (N) protein in B6.PL Thy1(a)/Cy mice (H-2(b)) immunized with plasmid DNA or recombinant vaccinia virus expressing SNV N protein were examined. Four peptides, NC94-101, NC175-189, NC217-231 and NC331-345, were recognized by CD8(+) T cells in CTL and ELISPOT assays in SNV N-immunized mice. Interestingly, two of these epitopes are located in the central region of the SNV N protein, where several human CD8(+) T-cell epitopes have been defined in Puumala virus and SNV. CTL lines specific for these four epitopes were cross-reactive to corresponding Puumala virus peptides, but only one of them was cross-reactive to Hantaan virus peptides. These results will enable the analysis of the roles of CTL in immunopathology of HPS in experimental mouse models of HPS.</p>
dc.identifier.submissionpathoapubs/1071
dc.contributor.departmentCenter for Infectious Disease and Vaccine Research
dc.source.pages1909-19


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