Flavivirus-cross-reactive, HLA-DR15-restricted epitope on NS3 recognized by human CD4+ CD8- cytotoxic T lymphocyte clones
UMass Chan AffiliationsDepartment of Medicine, Division of Infectious Diseases and Immunology
KeywordsAmino Acid Sequence
Molecular Sequence Data
Receptors, Antigen, T-Cell, alpha-beta
Sequence Homology, Amino Acid
Viral Nonstructural Proteins
Medicine and Health Sciences
MetadataShow full item record
AbstractThe role of flavivirus-cross-reactive T lymphocytes in recovery from and pathogenesis of flavivirus infections is not known. In the present paper, we have defined a flavivirus-cross-reactive epitope recognized by two CD4+ CD8- cytotoxic T lymphocyte (CTL) clones, JK4 and JK43. The T cell clones were established from the peripheral blood T lymphocytes of a dengue-4-immune donor, using a limiting-dilution method with dengue-4 antigen. These two T cell clones were cross-reactive for dengue virus types 1, 2, 3 and 4, yellow fever virus and West Nile virus, and recognized NS3 protein. The smallest synthetic peptide recognized by these T cell clones was an identical 9 amino acid peptide which contains amino acids 146 to 154 (VIGLYGNGV) of dengue-4 NS3. HLA-DR15 was the restriction allele for recognition of this epitope by JK4 and JK43. JK4 and JK43 both used T cell receptor V alpha 8, but JK4 used V beta 8 and JK43 used V beta 2. This result indicates that this epitope is recognized by two flavivirus-cross-reactive CD4+ T cell clones which originated from different T cells in vivo.
J Gen Virol. 1995 Sep;76 ( Pt 9):2243-9.
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/38195