Direct visualization of cross-reactive effector and memory allo-specific CD8 T cells generated in response to viral infections
AuthorsBrehm, Michael A.
Markees, Thomas G.
Daniels, Keith A.
Greiner, Dale L.
Rossini, Aldo A.
Welsh, Raymond M.
*Cytotoxicity Tests, Immunologic
Interferon Type II
Lymphocytic choriomeningitis virus
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Inbred CBA
Mice, Inbred DBA
Receptors, Antigen, T-Cell, alpha-beta
Tumor Cells, Cultured
Medicine and Health Sciences
MetadataShow full item record
AbstractCD8 T cell cross-reactivity between heterologous viruses has been shown to provide protective immunity, induce immunopathology, influence the immunodominance of epitope-specific T cell responses, and shape the overall memory population. Virus infections also induce cross-reactive allo-specific CTL responses. In this study, we quantified the allo-specific CD8 T cells elicited by infection of C57BL/6 (B6) mice with lymphocytic choriomeningitis virus (LCMV). Cross-reactive LCMV-specific CD8 T cells were directly visualized using LCMV peptide-charged MHC tetramers to costain T cells that were stimulated to produce intracellular IFN-gamma in response to allogeneic target cells. The cross-reactivity between T cells specific for LCMV and allogeneic Ags was broad-based, in that it involved multiple LCMV-derived peptides, but there were distinctive patterns of reactivity against allogeneic cells with different haplotypes. Experiments indicated that this cross-reactivity was not due to the expression of two TCR per cell, and that the patterns of allo-reactivity changed during sequential infection with heterologous viruses. The allo-specific CD8 T cells generated by LCMV infection were maintained at relatively high frequencies in the memory pool, indicating that memory allo-specific CD8 T cell populations can arise as a consequence of viral infections. Mice previously infected with LCMV and harboring allo-specific memory T cells were refractory to the induction of tolerance to allogeneic skin grafts.
J Immunol. 2003 Apr 15;170(8):4077-86.
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/38238
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