Differential kinetics and specificity of EBV-specific CD4+ and CD8+ T cells during primary infection
AuthorsPrecopio, Melissa Lynn
Sullivan, John L.
Document TypeJournal Article
Amino Acid Sequence
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Epstein-Barr Virus Nuclear Antigens
Herpesvirus 4, Human
Molecular Sequence Data
Medicine and Health Sciences
MetadataShow full item record
AbstractThe generation and maintenance of virus-specific CD4(+) T cells in humans are not well understood. We used short in vitro stimulation assays followed by intracellular cytokine staining to characterize the timing, magnitude, and Ag specificity of CD4(+) T cells over the course of primary EBV infection. Lytic and latent protein-specific CD4(+) T cells were readily detected at presentation with acute infectious mononucleosis and declined rapidly thereafter. Responses to BZLF-1, BMLF-1, and Epstein-Barr nuclear Ag-3A were more commonly detected than responses to Epstein-Barr nuclear Ag-1. Concurrent analyses of BZLF-1-specific CD4(+) and CD8(+) T cells revealed differences in the expansion, specificity, and stability of CD4(+) and CD8(+) T cell-mediated responses over time. Peripheral blood EBV load directly correlated with the frequency of EBV-specific CD4(+) T cell responses at presentation and over time, suggesting that EBV-specific CD4(+) T cell responses are Ag-driven.
J Immunol. 2003 Mar 1;170(5):2590-8.
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/38242