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dc.contributor.authorZivna, Iva
dc.contributor.authorGreen, Sharone
dc.contributor.authorVaughn, David W.
dc.contributor.authorKalayanarooj, Siripen
dc.contributor.authorStephens, Henry A. F.
dc.contributor.authorChandanayingyong, Dasnayanee
dc.contributor.authorNisalak, Ananda
dc.contributor.authorEnnis, Francis A.
dc.contributor.authorRothman, Alan L.
dc.date2022-08-11T08:09:31.000
dc.date.accessioned2022-08-23T16:34:03Z
dc.date.available2022-08-23T16:34:03Z
dc.date.issued2002-05-23
dc.date.submitted2009-03-10
dc.identifier.citation<p>J Immunol. 2002 Jun 1;168(11):5959-65.</p>
dc.identifier.issn0022-1767 (Print)
dc.identifier.doi10.4049/jimmunol.168.11.5959
dc.identifier.pmid12023403
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38246
dc.description.abstractDengue hemorrhagic fever (DHF), the severe manifestation of dengue virus (DV) infection characterized by plasma leakage, is more common in secondary DV infections in previously infected individuals and is associated with high levels of immune activation. To determine the Ag specificity of this immune response, we studied the response to an HLA-B*07-restricted T cell epitope, residues 221-232 of the DV NS3 protein, in 10 HLA-B*07(+) Thai children who were studied during and after acute DV infections. Peptide-specific T cells were detected in 9 of 10 subjects. The frequency of peptide-specific T cells was higher in subjects who had experienced DHF than in those who had experienced DF. We also detected peptide-specific T cells in PBMC obtained at the time of the acute DV infection in 2 of 5 subjects. These data suggest that the NS3 (221-232) epitope is an important target of CD8(+) T cells in secondary DV infection and that the activation and expansion of DV-specific T cells is greater in subjects with DHF than in those with dengue fever. These findings support the hypothesis that activation of DV-specific CD8(+) T cells plays an important role in the pathogenesis of DHF.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=12023403&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.4049/jimmunol.168.11.5959
dc.subjectAdolescent
dc.subjectChild
dc.subjectChild, Preschool
dc.subjectDengue
dc.subject*Epitopes, T-Lymphocyte
dc.subjectHLA-B Antigens
dc.subjectHumans
dc.subjectInfant
dc.subjectInterleukin-2
dc.subjectPeptide Fragments
dc.subjectProspective Studies
dc.subjectRNA Helicases
dc.subjectRNA, Viral
dc.subjectSerine Endopeptidases
dc.subjectT-Lymphocytes
dc.subjectViral Nonstructural Proteins
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleT cell responses to an HLA-B*07-restricted epitope on the dengue NS3 protein correlate with disease severity
dc.typeJournal Article
dc.source.journaltitleJournal of immunology (Baltimore, Md. : 1950)
dc.source.volume168
dc.source.issue11
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/1120
dc.identifier.contextkey770098
html.description.abstract<p>Dengue hemorrhagic fever (DHF), the severe manifestation of dengue virus (DV) infection characterized by plasma leakage, is more common in secondary DV infections in previously infected individuals and is associated with high levels of immune activation. To determine the Ag specificity of this immune response, we studied the response to an HLA-B*07-restricted T cell epitope, residues 221-232 of the DV NS3 protein, in 10 HLA-B*07(+) Thai children who were studied during and after acute DV infections. Peptide-specific T cells were detected in 9 of 10 subjects. The frequency of peptide-specific T cells was higher in subjects who had experienced DHF than in those who had experienced DF. We also detected peptide-specific T cells in PBMC obtained at the time of the acute DV infection in 2 of 5 subjects. These data suggest that the NS3 (221-232) epitope is an important target of CD8(+) T cells in secondary DV infection and that the activation and expansion of DV-specific T cells is greater in subjects with DHF than in those with dengue fever. These findings support the hypothesis that activation of DV-specific CD8(+) T cells plays an important role in the pathogenesis of DHF.</p>
dc.identifier.submissionpathoapubs/1120
dc.contributor.departmentDepartment of Medicine, Division of Infectious Diseases and Immunology
dc.contributor.departmentCenter for Infectious Disease and Vaccine Research
dc.source.pages5959-65


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