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Pertussis toxin blocks the inhibitory effects of calcitonin on cyclic AMP accumulation in stimulated cultured human monocytes

dc.contributor.authorStock, John L.
dc.contributor.authorCoderre, James A.
dc.date2022-08-11T08:09:31.000
dc.date.accessioned2022-08-23T16:34:12Z
dc.date.available2022-08-23T16:34:12Z
dc.date.issued1987-11-01
dc.date.submitted2009-03-10
dc.identifier.citation<p>J Leukoc Biol. 1987 Nov;42(5):504-9.</p>
dc.identifier.issn0741-5400 (Print)
dc.identifier.doi10.1002/jlb.42.5.504
dc.identifier.pmid2824646
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38279
dc.description.abstractSurface stimulation of fresh or cultured human mononuclear cells by latex particles causes an increase in the accumulation of cyclic AMP that is inhibited by preincubation with calcitonin (CT). Preincubation of cultured monocytes with 500 ng/ml pertussis toxin totally blocks the inhibitory effects of CT at low concentrations of this hormone. The effects of pertussis toxin are dose-related and eliminated by boiling the toxin. Similar preincubations with cholera toxin have no significant effects on subsequent inhibition of surface-stimulated cyclic AMP by CT. Membranes prepared from cultured human monocytes contain a 41,000-dalton protein that is ADP-ribosylated by pertussis toxin and may be the inhibitory guanine nucleotide regulatory protein (Ni) mediating this inhibition.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=2824646&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1002/jlb.42.5.504
dc.subjectAdenosine Diphosphate Ribose
dc.subjectCalcitonin
dc.subjectCell Membrane
dc.subjectCells, Cultured
dc.subjectCholera Toxin
dc.subjectCyclic AMP
dc.subjectHeat
dc.subjectHumans
dc.subjectMonocytes
dc.subject*Pertussis Toxin
dc.subjectVirulence Factors, Bordetella
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titlePertussis toxin blocks the inhibitory effects of calcitonin on cyclic AMP accumulation in stimulated cultured human monocytes
dc.typeJournal Article
dc.source.journaltitleJournal of leukocyte biology
dc.source.volume42
dc.source.issue5
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/1151
dc.identifier.contextkey770129
html.description.abstract<p>Surface stimulation of fresh or cultured human mononuclear cells by latex particles causes an increase in the accumulation of cyclic AMP that is inhibited by preincubation with calcitonin (CT). Preincubation of cultured monocytes with 500 ng/ml pertussis toxin totally blocks the inhibitory effects of CT at low concentrations of this hormone. The effects of pertussis toxin are dose-related and eliminated by boiling the toxin. Similar preincubations with cholera toxin have no significant effects on subsequent inhibition of surface-stimulated cyclic AMP by CT. Membranes prepared from cultured human monocytes contain a 41,000-dalton protein that is ADP-ribosylated by pertussis toxin and may be the inhibitory guanine nucleotide regulatory protein (Ni) mediating this inhibition.</p>
dc.identifier.submissionpathoapubs/1151
dc.contributor.departmentEndocrinology Research Laboratory
dc.source.pages504-9


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