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    A comparison in monkeys of (99m)Tc labeled to a peptide by 4 methods

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    Authors
    Rusckowski, Mary
    Qu, Tong
    Gupta, Suresh
    Ley, Arthur C.
    Hnatowich, Donald J.
    UMass Chan Affiliations
    Department of Radiology, Division of Nuclear Medicine
    Document Type
    Journal Article
    Publication Date
    2001-12-26
    Keywords
    Animals
    Chelating Agents
    Isotope Labeling
    Leukocyte Elastase
    Macaca mulatta
    Male
    Mice
    Peptides
    Staphylococcal Infections
    Technetium
    Tissue Distribution
    Life Sciences
    Medicine and Health Sciences
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    Link to Full Text
    http://jnm.snmjournals.org/content/42/12/1870.full.pdf+html
    Abstract
    Although a number of different strategies for labeling peptides with (99m)Tc have been developed, only a few studies have compared the in vivo properties of (99m)Tc when attached to different chelators. Furthermore, these comparisons are usually in mice, whereas results obtained in nonhuman primates may be expected to be more relevant to the clinical situation. METHODS: We evaluated the influence of 4 common chelators on the biodistribution in monkeys of (99m)Tc-labeled HNE-2, a 6.7-kDa peptide being investigated as an inflammation/infection imaging agent. The peptide was conjugated with the N-hydroxysuccinimide ester of mercaptoacetyltriglycine (MAG3), mercaptoacetyltriserine (MAS3), hydrazinonicotinamide (HYNIC), and the cyclic anhydride of diethylenetriaminepentaacetic acid (DTPA). After radiolabeling, each peptide was administered intravenously to rhesus monkeys with a Staphylococcus aureus-induced focal inflammation/infection. RESULTS: Quantification of radioactivity accumulation by regions of interest over 3 h after administration in monkeys showed important differences among labeling methods: For example, at 3 h, kidney accumulation varied in percentage injected dose per organ (%ID per organ) from 31 %ID per organ (HYNIC) to 18 %ID per organ (MAG3), whereas liver varied from 7.8 %ID per organ (MAG3) to 2.8 %ID per organ (MAS3). Radioactivity accumulation in the lesion was independent of labeling method. These organ accumulations were compared with that obtained earlier in mice by sacrifice and dissection also at 3 h and at the same administered dosage. In the rodent, kidney levels varied from 45 %ID per organ (HYNIC) to 12 %ID per organ (MAS3) and liver levels varied from 6.5 %ID per organ (DTPA) to 2.0 %ID per organ (MAS3). CONCLUSION: In agreement with previous work from this laboratory and elsewhere, the method of radiolabeling had an important effect on the biodistribution of (99m)Tc. Furthermore, although biodistribution results in mice should be used with caution to predict biodistributions in primates, in major organs, these results in mice and monkeys were similar.
    Source
    J Nucl Med. 2001 Dec;42(12):1870-7.
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/38322
    PubMed ID
    11752087
    Related Resources
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    UMass Chan Faculty and Researcher Publications
    Radiology Publications

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