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dc.contributor.authorLiu, Guozheng
dc.contributor.authorDou, Shuping
dc.contributor.authorYin, Dongguang
dc.contributor.authorSquires, Shayne
dc.contributor.authorLiu, Xinrong
dc.contributor.authorWang, Yi
dc.contributor.authorRusckowski, Mary
dc.contributor.authorHnatowich, Donald J.
dc.date2022-08-11T08:09:32.000
dc.date.accessioned2022-08-23T16:34:38Z
dc.date.available2022-08-23T16:34:38Z
dc.date.issued2007-04-28
dc.date.submitted2009-03-16
dc.identifier.citation<p>Cancer Biother Radiopharm. 2007 Feb;22(1):33-9. <a href="http://dx.doi.org/10.1089/cbr.2006.339">Link to article on publisher's site</a></p>
dc.identifier.issn1084-9785 (Print)
dc.identifier.doi10.1089/cbr.2006.339
dc.identifier.pmid17461727
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38381
dc.description.abstractA novel pretargeting method has been developed to quantitate antibody cellular internalization. In this study, the antibody was conjugated with a phosphorodiamidate morpholino oligomer (MORF) specific for the complementary MORF (cMORF) as an effector. Half the tumor cells were incubated with the MORF-antibody (pretargeting group) and the other half with the same MORF-antibody at the same concentration but radiolabeled (direct targeting group). After incubation, the same dosage of radiolabeled cMORF was added to the wells of the pretargeting group. The radioactivity of the direct targeting cells represented the sum of both internalized and cell-surface-bound antibodies, whereas the radioactivity of the pretargeting cells resulted only from the surface-bound antibodies, as the radiolabeled cMORF does not penetrate the cell surface. Therefore, the difference in radioactivity accumulation between pretargeting and direct targeting provides the internalized fraction. In this example, the internalization of a MORF conjugated anti-prostate-specific membrane antigen antibody, 3C6, in LNCaP cells was examined, and the average cell-surface residence time was determined as 2 hours. This method of measuring antibody internalization is directly applicable to pretargeting applications but can be a universal alternative to the conventional acid-wash method, with the advantage of leaving the cell membrane undamaged.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=17461727&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1949413/
dc.subjectAcids
dc.subjectAnimals
dc.subjectAntibodies
dc.subjectCell Line, Tumor
dc.subjectCell Membrane
dc.subjectDrug Delivery Systems
dc.subjectFlow Cytometry
dc.subjectHumans
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectMice, Nude
dc.subjectNeoplasms
dc.subjectXenograft Model Antitumor Assays
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleA novel pretargeting method for measuring antibody internalization in tumor cells
dc.typeJournal Article
dc.source.journaltitleCancer biotherapy and radiopharmaceuticals
dc.source.volume22
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/1245
dc.identifier.contextkey782912
html.description.abstract<p>A novel pretargeting method has been developed to quantitate antibody cellular internalization. In this study, the antibody was conjugated with a phosphorodiamidate morpholino oligomer (MORF) specific for the complementary MORF (cMORF) as an effector. Half the tumor cells were incubated with the MORF-antibody (pretargeting group) and the other half with the same MORF-antibody at the same concentration but radiolabeled (direct targeting group). After incubation, the same dosage of radiolabeled cMORF was added to the wells of the pretargeting group. The radioactivity of the direct targeting cells represented the sum of both internalized and cell-surface-bound antibodies, whereas the radioactivity of the pretargeting cells resulted only from the surface-bound antibodies, as the radiolabeled cMORF does not penetrate the cell surface. Therefore, the difference in radioactivity accumulation between pretargeting and direct targeting provides the internalized fraction. In this example, the internalization of a MORF conjugated anti-prostate-specific membrane antigen antibody, 3C6, in LNCaP cells was examined, and the average cell-surface residence time was determined as 2 hours. This method of measuring antibody internalization is directly applicable to pretargeting applications but can be a universal alternative to the conventional acid-wash method, with the advantage of leaving the cell membrane undamaged.</p>
dc.identifier.submissionpathoapubs/1245
dc.contributor.departmentDepartment of Radiology, Division of Nuclear Medicine
dc.source.pages33-9


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