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Phosphoinositide-dependent pathways in mouse sperm are regulated by egg ZP3 and drive the acrosome reaction

dc.contributor.authorJungnickel, Melissa K.
dc.contributor.authorSutton, Keith A.
dc.contributor.authorWang, Yanli
dc.contributor.authorFlorman, Harvey M.
dc.date2022-08-11T08:09:32.000
dc.date.accessioned2022-08-23T16:34:40Z
dc.date.available2022-08-23T16:34:40Z
dc.date.issued2007-01-30
dc.date.submitted2009-03-16
dc.identifier.citation<p>Dev Biol. 2007 Apr 1;304(1):116-26. Epub 2006 Dec 15. <a href="http://dx.doi.org/10.1016/j.ydbio.2006.12.023">Link to article on publisher's site</a></p>
dc.identifier.issn0012-1606 (Print)
dc.identifier.doi10.1016/j.ydbio.2006.12.023
dc.identifier.pmid17258189
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38386
dc.description.abstractSperm of many animals must complete an exocytotic event, the acrosome reaction, in order to fuse with eggs. In mammals, acrosome reactions are triggered during sperm contact with the egg extracellular matrix, or zona pellucida, by the matrix glycoprotein ZP3. Here, we show that ZP3 stimulates production of phosphatidylinositol-(3,4,5)-triphosphate in sperm membranes. Phosphatidylinositol-3-kinase antagonists that prevent acrosome reactions and fertilization in vitro, while generation of this phosphoinositide in the absence of ZP3 triggered acrosome reactions. Downstream effectors of phosphatidylinositol-(3,4,5)-triphosphate in sperm include the protein kinases, Akt and PKCzeta. These studies outline a signal transduction pathway that plays an essential role in the early events of mammalian fertilization.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=17258189&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1892180/
dc.subjectAcrosome Reaction
dc.subjectAnimals
dc.subjectEgg Proteins
dc.subjectFemale
dc.subjectFluorescent Antibody Technique
dc.subjectImmunoblotting
dc.subjectLipids
dc.subjectMale
dc.subjectMembrane Glycoproteins
dc.subjectMice
dc.subjectPhosphatidylinositol Phosphates
dc.subjectReceptors, Cell Surface
dc.subjectSignal Transduction
dc.subjectSpermatozoa
dc.subjectCell Biology
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titlePhosphoinositide-dependent pathways in mouse sperm are regulated by egg ZP3 and drive the acrosome reaction
dc.typeJournal Article
dc.source.journaltitleDevelopmental biology
dc.source.volume304
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/1251
dc.identifier.contextkey782918
html.description.abstract<p>Sperm of many animals must complete an exocytotic event, the acrosome reaction, in order to fuse with eggs. In mammals, acrosome reactions are triggered during sperm contact with the egg extracellular matrix, or zona pellucida, by the matrix glycoprotein ZP3. Here, we show that ZP3 stimulates production of phosphatidylinositol-(3,4,5)-triphosphate in sperm membranes. Phosphatidylinositol-3-kinase antagonists that prevent acrosome reactions and fertilization in vitro, while generation of this phosphoinositide in the absence of ZP3 triggered acrosome reactions. Downstream effectors of phosphatidylinositol-(3,4,5)-triphosphate in sperm include the protein kinases, Akt and PKCzeta. These studies outline a signal transduction pathway that plays an essential role in the early events of mammalian fertilization.</p>
dc.identifier.submissionpathoapubs/1251
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages116-26


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