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    Nonmuscle myosin IIb is involved in the guidance of fibroblast migration

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    Authors
    Lo, Chun-Min
    Buxton, Denis B.
    Chua, Gregory C. H.
    Dembo, Micah
    Adelstein, Robert S.
    Wang, Yu-Li
    UMass Chan Affiliations
    Department of Physiology
    Document Type
    Journal Article
    Publication Date
    2003-12-31
    Keywords
    Acrylic Resins
    Animals
    Cell Movement
    Cells, Cultured
    Embryo, Mammalian
    Fibroblasts
    Mice
    Mutation
    Nonmuscle Myosin Type IIB
    Life Sciences
    Medicine and Health Sciences
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    Abstract
    Although myosin II is known to play an important role in cell migration, little is known about its specific functions. We have addressed the function of one of the isoforms of myosin II, myosin IIB, by analyzing the movement and mechanical characteristics of fibroblasts where this protein has been ablated by gene disruption. Myosin IIB null cells displayed multiple unstable and disorganized protrusions, although they were still able to generate a large fraction of traction forces when cultured on flexible polyacrylamide substrates. However, the traction forces were highly disorganized relative to the direction of cell migration. Analysis of cell migration patterns indicated an increase in speed and decrease in persistence, which were likely responsible for the defects in directional movements as demonstrated with Boyden chambers. In addition, unlike control cells, mutant cells failed to respond to mechanical signals such as compressing forces and changes in substrate rigidity. Immunofluorescence staining indicated that myosin IIB was localized preferentially along stress fibers in the interior region of the cell. Our results suggest that myosin IIB is involved not in propelling but in directing the cell movement, by coordinating protrusive activities and stabilizing the cell polarity.
    Source
    Mol Biol Cell. 2004 Mar;15(3):982-9. Epub 2003 Dec 29. Link to article on publisher's site
    DOI
    10.1091/mbc.E03-06-0359
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/38513
    PubMed ID
    14699073
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1091/mbc.E03-06-0359
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    UMass Chan Faculty and Researcher Publications

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