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dc.contributor.authorMoen, Phillip T.
dc.contributor.authorJohnson, Carol V.
dc.contributor.authorByron, Meg
dc.contributor.authorShopland, Lindsay S.
dc.contributor.authorde la Serna, Ivana L.
dc.contributor.authorImbalzano, Anthony N.
dc.contributor.authorLawrence, Jeanne B.
dc.date2022-08-11T08:09:33.000
dc.date.accessioned2022-08-23T16:35:15Z
dc.date.available2022-08-23T16:35:15Z
dc.date.issued2003-11-18
dc.date.submitted2009-03-24
dc.identifier.citationMol Biol Cell. 2004 Jan;15(1):197-206. Epub 2003 Nov 14. <a href="http://dx.doi.org/10.1091/mbc.E03-06-0388">Link to article on publisher's site</a>
dc.identifier.issn1059-1524 (Print)
dc.identifier.doi10.1091/mbc.E03-06-0388
dc.identifier.pmid14617810
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38515
dc.description.abstractPrevious studies have shown that in a given cell type, certain active genes associate with SC-35 domains, nuclear regions rich in RNA metabolic factors and excluded from heterochromatin. This organization is not seen for all active genes; therefore, it is important to determine whether and when this locus-specific organization arises during development and differentiation of specific cell types. Here, we investigate whether gene organization relative to SC-35 domains is cell type specific by following several muscle and nonmuscle genes in human fibroblasts, committed but proliferative myoblasts, and terminally differentiated muscle. Although no change was seen for other loci, two muscle genes (Human beta-cardiac myosin heavy chain and myogenin) became localized to the periphery of an SC-35 domain in terminally differentiated muscle nuclei, but not in proliferative myoblasts or in fibroblasts. There was no apparent change in gene localization relative to either the chromosome territory or the heterochromatic compartment; thus, the gene repositioning seemed to occur specifically with respect to SC-35 domains. This gene relocation adjacent to a prominent SC-35 domain was recapitulated in mouse 3T3 cells induced into myogenesis by introduction of MyoD. Results demonstrate a cell type-specific reorganization of specific developmentally regulated loci relative to large domains of RNA metabolic factors, which may facilitate developmental regulation of genome expression.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=14617810&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectAnimals
dc.subjectCell Differentiation
dc.subjectCell Nucleus
dc.subjectCells, Cultured
dc.subjectChick Embryo
dc.subjectChromatin
dc.subjectHumans
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectMice
dc.subjectMicroscopy, Fluorescence
dc.subjectMuscle Development
dc.subjectMyoD Protein
dc.subjectMyoblasts
dc.subjectMyogenin
dc.subjectNIH 3T3 Cells
dc.subjectProtein Subunits
dc.subjectVentricular Myosins
dc.subjectCell Biology
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleRepositioning of muscle-specific genes relative to the periphery of SC-35 domains during skeletal myogenesis
dc.typeJournal Article
dc.source.journaltitleMolecular biology of the cell
dc.source.volume15
dc.source.issue1
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2387&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/1388
dc.identifier.contextkey794885
refterms.dateFOA2022-08-23T16:35:15Z
html.description.abstract<p>Previous studies have shown that in a given cell type, certain active genes associate with SC-35 domains, nuclear regions rich in RNA metabolic factors and excluded from heterochromatin. This organization is not seen for all active genes; therefore, it is important to determine whether and when this locus-specific organization arises during development and differentiation of specific cell types. Here, we investigate whether gene organization relative to SC-35 domains is cell type specific by following several muscle and nonmuscle genes in human fibroblasts, committed but proliferative myoblasts, and terminally differentiated muscle. Although no change was seen for other loci, two muscle genes (Human beta-cardiac myosin heavy chain and myogenin) became localized to the periphery of an SC-35 domain in terminally differentiated muscle nuclei, but not in proliferative myoblasts or in fibroblasts. There was no apparent change in gene localization relative to either the chromosome territory or the heterochromatic compartment; thus, the gene repositioning seemed to occur specifically with respect to SC-35 domains. This gene relocation adjacent to a prominent SC-35 domain was recapitulated in mouse 3T3 cells induced into myogenesis by introduction of MyoD. Results demonstrate a cell type-specific reorganization of specific developmentally regulated loci relative to large domains of RNA metabolic factors, which may facilitate developmental regulation of genome expression.</p>
dc.identifier.submissionpathoapubs/1388
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages197-206


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