The Drosophila kinesin-like protein KLP67A is essential for mitotic and male meiotic spindle assembly
Authors
Gandhi, RitaBonaccorsi, Silvia
Wentworth, Diana
Doxsey, Stephen J.
Gatti, Maurizio
Pereira, Andrea J.
UMass Chan Affiliations
Program in Molecular MedicineDepartment of Molecular Genetics and Microbiology
Document Type
Journal ArticlePublication Date
2003-09-19Keywords
AnimalsCentrosome
Chromosome Segregation
Drosophila
Drosophila Proteins
Genes, Fungal
Male
Meiosis
Microscopy, Fluorescence
Microtubule-Associated Proteins
Microtubules
Mitosis
Mitotic Spindle Apparatus
Mutation
RNA, Small Interfering
Spermatocytes
Tubulin
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
We have performed a mutational analysis together with RNA interference to determine the role of the kinesin-like protein KLP67A in Drosophila cell division. During both mitosis and male meiosis, Klp67A mutations cause an increase in MT length and disrupt discrete aspects of spindle assembly, as well as cytokinesis. Mutant cells exhibit greatly enlarged metaphase spindle as a result of excessive MT polymerization. The analysis of both living and fixed cells also shows perturbations in centrosome separation, chromosome segregation, and central spindle assembly. These data demonstrate that the MT plus end-directed motor KLP67A is essential for spindle assembly during mitosis and male meiosis and suggest that the regulation of MT plus-end polymerization is a key determinant of spindle architecture throughout cell division.Source
Mol Biol Cell. 2004 Jan;15(1):121-31. Epub 2003 Sep 17. Link to article on publisher's siteDOI
10.1091/mbc.E03-05-0342Permanent Link to this Item
http://hdl.handle.net/20.500.14038/38516PubMed ID
13679514Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1091/mbc.E03-05-0342