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    The unique catalytic subunit of sperm cAMP-dependent protein kinase is the product of an alternative Calpha mRNA expressed specifically in spermatogenic cells

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    Authors
    San Agustin, Jovenal T.
    Wilkerson, Curtis G.
    Witman, George B.
    UMass Chan Affiliations
    Program in Molecular Medicine
    Department of Cell Biology
    Document Type
    Journal Article
    Publication Date
    2000-09-12
    Keywords
    *Alternative Splicing
    Amino Acid Sequence
    Animals
    Base Sequence
    Brain
    Catalytic Domain
    Cattle
    Cloning, Molecular
    Consensus Sequence
    Cyclic AMP-Dependent Protein Kinases
    Humans
    Isoenzymes
    Male
    Mice
    Molecular Sequence Data
    Protein Subunits
    Rats
    Recombinant Proteins
    Sequence Alignment
    Sequence Homology, Amino Acid
    Sheep
    Spermatogenesis
    Spermatozoa
    Testis
    Life Sciences
    Medicine and Health Sciences
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    Abstract
    cAMP-dependent protein kinase has a central role in the control of mammalian sperm capacitation and motility. Previous protein biochemical studies indicated that the only cAMP-dependent protein kinase catalytic subunit (C) in ovine sperm is an unusual isoform, termed C(s), whose amino terminus differs from those of published C isoforms of other species. Isolation and sequencing of cDNA clones encoding ovine C(s) and Calpha1 (the predominant somatic isoform) now reveal that C(s) is the product of an alternative transcript of the Calpha gene. C(s) cDNA clones from murine and human testes also were isolated and sequenced, indicating that C(s) is of ancient origin and widespread in mammals. In the mouse, C(s) transcripts were detected only in testis and not in any other tissue examined, including ciliated tissues and ovaries. Finally, immunohistochemistry of the testis shows that C(s) first appears in pachytene spermatocytes. This is the first demonstration of a cell type-specific expression for any C isoform. The conservation of C(s) throughout mammalian evolution suggests that the unique structure of C(s) is important in the subunit's localization or function within the sperm.
    Source
    Mol Biol Cell. 2000 Sep;11(9):3031-44.
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/38524
    PubMed ID
    10982398
    Related Resources
    Link to Article in PubMed
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    UMass Chan Faculty and Researcher Publications
    Witman Lab

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