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dc.contributor.authorWheatley, Sally P.
dc.contributor.authorO'Connell, Christopher B.
dc.contributor.authorWang, Yu-Li
dc.date2022-08-11T08:09:33.000
dc.date.accessioned2022-08-23T16:35:20Z
dc.date.available2022-08-23T16:35:20Z
dc.date.issued1998-08-07
dc.date.submitted2009-03-24
dc.identifier.citationMol Biol Cell. 1998 Aug;9(8):2173-84.
dc.identifier.issn1059-1524 (Print)
dc.identifier.pmid9693374
dc.identifier.urihttp://hdl.handle.net/20.500.14038/38534
dc.description.abstractWhile astral microtubules are believed to be primarily responsible for the stimulation of cytokinesis in Echinoderm embryos, it has been suggested that a signal emanating from the chromosomal region and mediated by the interzonal microtubules stimulates cytokinesis in cultured mammalian cells. To test this hypothesis, we examined cytokinesis in normal rat kidney cells treated with an inhibitor of topoisomerase II, (+)-1,2-bis(3,5-dioxopiperaz-inyl-1-yl)propane, which prevents the separation of sister chromatids and the formation of a spindle interzone. The majority of treated cells showed various degrees of abnormality in cytokinesis. Furrows frequently deviated from the equatorial plane, twisting daughter cells into irregular shapes. Some cells developed furrows in regions outside the equator or far away from the spindle. In addition, F-actin and myosin II accumulated at the lateral ingressing margins but did not form a continuous band along the equator as in control cells. Imaging of microinjected 5- (and 6-) carboxymtetramethylrhodamine-tubulin revealed that a unique set of microtubules projected out from the chromosomal vicinity upon anaphase onset. These microtubules emanated toward the lateral cortex, where they delineated sites of microtubule bundle formation, cortical ingression, and F-actin and myosin II accumulation. As centrosome integrity and astral microtubules appeared unperturbed by (+)-1,2-bis(3, 5-dioxopiperaz-inyl-1-yl)propane treatment, the present observations cannot be easily explained by the conventional model involving astral microtubules. We suggest that in cultured epithelial cells the organization of the chromosomes dictates the organization of midzone microtubules, which in turn determines and maintains the cleavage activity.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=9693374&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectActins
dc.subjectAnimals
dc.subjectCell Division
dc.subjectCell Line
dc.subjectChromosomes
dc.subjectDNA Topoisomerases, Type II
dc.subjectEpithelial Cells
dc.subjectKidney
dc.subjectMicrotubules
dc.subjectModels, Biological
dc.subjectMyosins
dc.subjectRats
dc.subjectRazoxane
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleInhibition of chromosomal separation provides insights into cleavage furrow stimulation in cultured epithelial cells
dc.typeArticle
dc.source.journaltitleMolecular biology of the cell
dc.source.volume9
dc.source.issue8
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2403&amp;context=oapubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/1404
dc.identifier.contextkey794901
refterms.dateFOA2022-08-23T16:35:20Z
html.description.abstract<p>While astral microtubules are believed to be primarily responsible for the stimulation of cytokinesis in Echinoderm embryos, it has been suggested that a signal emanating from the chromosomal region and mediated by the interzonal microtubules stimulates cytokinesis in cultured mammalian cells. To test this hypothesis, we examined cytokinesis in normal rat kidney cells treated with an inhibitor of topoisomerase II, (+)-1,2-bis(3,5-dioxopiperaz-inyl-1-yl)propane, which prevents the separation of sister chromatids and the formation of a spindle interzone. The majority of treated cells showed various degrees of abnormality in cytokinesis. Furrows frequently deviated from the equatorial plane, twisting daughter cells into irregular shapes. Some cells developed furrows in regions outside the equator or far away from the spindle. In addition, F-actin and myosin II accumulated at the lateral ingressing margins but did not form a continuous band along the equator as in control cells. Imaging of microinjected 5- (and 6-) carboxymtetramethylrhodamine-tubulin revealed that a unique set of microtubules projected out from the chromosomal vicinity upon anaphase onset. These microtubules emanated toward the lateral cortex, where they delineated sites of microtubule bundle formation, cortical ingression, and F-actin and myosin II accumulation. As centrosome integrity and astral microtubules appeared unperturbed by (+)-1,2-bis(3, 5-dioxopiperaz-inyl-1-yl)propane treatment, the present observations cannot be easily explained by the conventional model involving astral microtubules. We suggest that in cultured epithelial cells the organization of the chromosomes dictates the organization of midzone microtubules, which in turn determines and maintains the cleavage activity.</p>
dc.identifier.submissionpathoapubs/1404
dc.contributor.departmentDepartment of Physiology
dc.source.pages2173-84


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