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    Human Slug is a repressor that localizes to sites of active transcription

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    Authors
    Hemavathy, Kirugaval
    Guru, S C
    Harris, John E.
    Chen, J. Don
    Ip, Y. Tony
    UMass Chan Affiliations
    Program in Molecular Medicine
    Document Type
    Journal Article
    Publication Date
    2000-06-24
    Keywords
    Base Sequence
    Binding Sites
    Cell Nucleus
    Chromatin
    Conserved Sequence
    DNA-Binding Proteins
    Enzyme Inhibitors
    Fluorescent Antibody Technique
    Histone Deacetylases
    Humans
    Hydroxamic Acids
    Molecular Sequence Data
    Neoplasm Proteins
    *Nuclear Proteins
    RNA Splicing
    Repressor Proteins
    Sequence Deletion
    Transcription Factors
    *Transcription, Genetic
    Tumor Suppressor Proteins
    Zinc Fingers
    Life Sciences
    Medicine and Health Sciences
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    Abstract
    Snail/Slug family proteins have been identified in diverse species of both vertebrates and invertebrates. The proteins contain four to six zinc fingers and function as DNA-binding transcriptional regulators. Various members of the family have been demonstrated to regulate cell movement, neural cell fate, left-right asymmetry, cell cycle, and apoptosis. However, the molecular mechanisms of how these regulators function and the target genes involved are largely unknown. In this report, we demonstrate that human Slug (hSlug) is a repressor and modulates both activator-dependent and basal transcription. The repression depends on the C-terminal DNA-binding zinc fingers and on a separable repression domain located in the N terminus. This domain may recruit histone deacetylases to modify the chromatin and effect repression. Protein localization study demonstrates that hSlug is present in discrete foci in the nucleus. This subnuclear pattern does not colocalize with the PML foci or the coiled bodies. Instead, the hSlug foci overlap extensively with areas of the SC-35 staining, some of which have been suggested to be sites of active splicing or transcription. These results lead us to postulate that hSlug localizes to target promoters, where activation occurs, to repress basal and activator-mediated transcription.
    Source
    Mol Cell Biol. 2000 Jul;20(14):5087-95.
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/38570
    PubMed ID
    10866665
    Related Resources
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