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    E2F4 actively promotes the initiation and maintenance of nerve growth factor-induced cell differentiation

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    Authors
    Persengiev, Stephan P.
    Kondova, Ivanela I.
    Kilpatrick, Daniel L.
    UMass Chan Affiliations
    Department of Cellular and Molecular Physiology
    Document Type
    Journal Article
    Publication Date
    1999-08-24
    Keywords
    Animals
    Base Sequence
    Cell Differentiation
    Cell Division
    Cell Nucleus
    Central Nervous System
    DNA-Binding Proteins
    E2F4 Transcription Factor
    Nerve Growth Factors
    Neurons
    Oligodeoxyribonucleotides, Antisense
    PC12 Cells
    Phosphoproteins
    Promoter Regions (Genetics)
    *Proteins
    Rats
    Retinoblastoma Protein
    Retinoblastoma-Like Protein p130
    Tetracycline
    Transcription Factors
    Up-Regulation
    Life Sciences
    Medicine and Health Sciences
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    Abstract
    E2F transcription factors play a critical role in cell cycle progression through the regulation of genes required for G(1)/S transition. They are also thought to be important for growth arrest; however, their potential role in the cell differentiation process has not been previously examined. Here, we demonstrate that E2F4 is highly upregulated following the neuronal differentiation of PC12 cells with nerve growth factor (NGF), while E2F1, E2F3, and E2F5 are downregulated. Immunoprecipitation and subcellular fractionation studies demonstrated that both the nuclear localization of E2F4 and its association with the Rb family member p130 increased following neuronal differentiation. The forced expression of E2F4 markedly enhanced the rate of PC12 cell differentiation induced by NGF and also greatly lowered the rate at which cells lost their neuronal phenotype following NGF removal. Importantly, this effect occurred in the absence of any significant change in the growth regulation of PC12 cells by NGF. Further, the downregulation of E2F4 expression with antisense oligodeoxynucleotides inhibited NGF-induced neurite outgrowth, indicating an important role for this factor during PC12 cell differentiation. Finally, E2F4 expression was found to increase dramatically in the developing rat cerebral cortex and cerebellum, as neuroblasts became postmitotic and initiated terminal differentiation. These findings demonstrate that, in addition to its effects on cell proliferation, E2F4 actively promotes the neuronal differentiation of PC12 cells as well as the retention of this state. Further, this effect is independent of alterations in cell growth and may involve interactions between E2F4 and the neuronal differentiation program itself. E2F4 may be an important participant in the terminal differentiation of neuroblasts.
    Source
    Mol Cell Biol. 1999 Sep;19(9):6048-56.
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/38577
    PubMed ID
    10454552
    Related Resources
    Link to Article in PubMed
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    UMass Chan Faculty and Researcher Publications

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